(×10)Fig4HT-29cellsextendgrowthaftertwodaysunderthemicroscope(×10)注:箭头为HT-29细胞图5HT-29细胞传代生长4日后显微镜下生长情况(×10)Fig5HT-29cellsextendgrowthafterfourdaysunderthemicroscope(×10)注:箭头为HT-29细胞2.2HT-29肿瘤细胞移植后其肿瘤及血管生长特征鸡胚胎动频繁,不能完全避免移植瘤生长位置...
[结论]应用慢病毒载体系统转染 Grb2 shRNA 至 HT29 细胞,导致 Grb2 在 mRNA 和蛋白水平上明显抑制,并影响HT29 细胞生长和相关信号转导通路分子表达下 调。 [关键词]结直肠癌 Grb2 RNA 干扰 慢病毒 信号通路 2 万方数据 Grb2 inhibition of colon cancer cells HT29 tumor-associated signal transduction ...
干扰HT-29细胞Livin基因的表达。HT-29细胞Livin基因抑制后,细胞增殖能 力减弱,细胞凋亡增加,细胞周期重新分布,能增强5.Fu、奥沙利铂和伊立替 康的抗肿瘤作用。现进一步通过体内实验探讨慢病毒载体介导RNA干扰沉默 Livin基因表达,对大肠癌HT-29细胞裸鼠移植瘤生长及对放化疗敏感性的影响, ...
emodinonhumancoloncancel"cellsRKO,HT一29,Caco-2invitro. Methods:Thisresearchselects poorly,moderately andwell—differentiatedofhumancolon carcinomacelllinesRKO,HT-29,andCaco,2.A1leelllinesWeretreatedwithemodinat 100,50,25,12.51aM ormedia.Treatmenttimewas24,48,and72hours.Therelative ...
1.Thedifferentiationofpheochromocytomacells(PC12)wasinducedbynerve growthfactor(NGF)toconstructtheneuralcellmodel. 2.WesternBlotwasusedtodetectthelevelofLAT1proteininPC12cellsto studytheeffectsofVDandestrogenonLAT1proteinexpression. 3.ELISAwasusedtodeterminetheconcentrationof5-HTinthesupernatantof PC12cells....