带绿色荧光蛋白(Greenfluorescentprotein,GFP)的重组型BAC—HSV-1感染性子代病毒。构建了携带HsV一1同 源臂的质粒C223一UI43左臂一uI47右臂。将该质粒线性化后与HSV-I基因组共转染至Vero细胞,通过真核细胞 内同源重组产生了含有GFP报告基因的BAC-HSV-1重组病毒,噬斑纯化筛选出阳性重组病毒,并再次感染Vero ...
本研究旨在构建由细菌人工染色体(Bacteria artificial chromosome,BAC)携带的单纯疱疹I型病毒质粒及携带绿色荧光蛋白(Green fluorescent protein,GFP)的重组型BAC-HSV-1感染性子代病毒.构建了携带HSV-1同源臂的质粒C223-UL43左臂-UL47右臂.将该质粒线性化后与HSV-1基因组共转染至Vero细胞,通过真核细胞内同源重组产生...
本研究旨在构建由细菌人工染色体(Bacteria artificial chromosome,BAC)携带的单纯疱疹I型病毒质粒及携带绿色荧光蛋白(Green fluorescent protein,GFP)的重组型BAC-HSV-1感染性子代病毒.构建了携带HSV-1同源臂的质粒C223-UL43左臂-UL47右臂.将该质粒线性化后与HSV-1基因组共转染至Vero细胞,通过真核细胞内同源重组产生了...
黑色素瘤目的建立具有绿色荧光蛋白(GFP)示踪功能的单纯疱疹病毒1型胸苷激酶基因/更昔洛韦自杀基因系统(HSV1-tkGFP/GCV),并检测该系统对小鼠黑色素瘤的抑制作用.方法将阳性重组质粒pLXSN-tkGFP和pLXSN-DsRed2分别转染B16细胞,加G418进行筛选.用GCV(5,50,500μmol·L-1)杀伤实验验证tk基因的表达.四甲基偶氮唑盐(...
3,16例同时经Cell Search和Ohsv1-HTERT-GFP法检测的胰腺癌患者的中位发病年龄为61岁,男性患者(68.8%)比例高于女性患者(31.1%).16例患者的中位无进展生存时间为6.1m,总生存时间为8.8m.Cell Search的CTC检出率为43.7%(7/16),Ohsv1-HTERT-GFP方法的检出率为87.5%(14/16).经Cell Search检测的基线CTC阳性...
Ponomarev V, Doubrovin M, Serganova I, Beresten T, Vider J, Shavrin A, Ageyeva L, Balatoni J, Blasberg R, Tjuvajev JG (2003) Cytoplasmically retargeted HSV1-tk/GFP reporter gene mutants for optimization of noninvasive molecular-genetic imaging. Neoplasia 5:245–254...
GFP-tagged ICP0 is predominantly cytoplasmic in HSV-1 infected cells.Mingyu, LiuEdward, E. SchmidtWilliam, P. Halford
Subcellular distribution of ICP0GFP in HSV-1 infected cells at times after release from a cycloheximide blocka.Mingyu, LiuEdward, E. SchmidtWilliam, P. Halford
APP and TGN46 do not co-localize with VP26-GFP labeled viral particles in the periphery of HSV1 infected cells: Evidence for specific APP-HSV1 interactions.ShiBin ChengPaulette FerlandPaul WebsterElaine L. Bearer