PCR grade water should be added to make up the final volume of the PCR reaction to 20 µL. • Using a lower volume from the sample than recommended may affect the sensitivity of the HSV-2 Limit of Detection. • An HSV-2 Negative Control (NegC) and HSV-2 Positive Control (PosC)...
False positive PCR was seen in 1993 and 1994, due to sample-to-sample contamination during division of samples, but was not seen since 1995 when this procedure was changed. The test results depended on the state of the disease. Thus, the sensitivity, specificity, PPV and NPV for ITT were...
RegisterLog in Sign up with one click: Facebook Twitter Google Share on Facebook AcronymDefinition HSVEHeated Soil Vapor Extraction HSVEHerpes Simplex Virus Esophagitis HSVEHuman Saphenous Vein Endothelial HSVEHome School Vacation Exchange(Logansport, IN) ...
nodes isolated on 4 days pi. Representative dot plots and quantification of the frequency of proliferating OT-I T cells in the presence (c) or absence (d) of the OVA peptide (SIINFEKL).N = 5–6 per group. The data are presented as mean ± SEM.Pvalues were obtained by usin...
The eluted DNA was quantified using a Nanodrop 2000 spectrophotometer and equal amounts of DNA were used to perform quantitative PCR using an Applied Biosystems 7500 Fast Real-Time PCR System. To maximize sensitivity, extracted DNA was evaluated at concentrations ranging from 100 to 800 ng. DNA...
Secreted herpes simplex virus-2 glycoprotein G alters thermal pain sensitivity by modifying NGF effects on TRPV1. J Neuroinflammation. 2016;13:210. 16. Schmittgen TD, Livak KJ. Analyzing real-time PCR data by the comparative C(T) method. Nat Protoc. 2008;3:1101–8. 17. Ding L, Jiang ...
vostudy.Ininvitrostudy.sensitivityofHelMTKcelstoGCVandbystanderefectwereobservedbyM'ITtest.Ininvivo study,Hela/TKcelsandHelacelswereinjecteds.c.intoflankingBALB/Cmice,GCVwasadministratedi.P.,RT-PCRwasapplied todetecttheexpressionofHSV-TKgene.StatisticalanalysisofthedatawasperformedbyusingtheANOVA(analysis...
but inclusion of samples with known genotypes in each ddPCR run permitted genotype assignment (examples,SI Fig. 1). One or bothUL39ddPCR assays failed for 9% of genital swab DNA specimens, correlating with low amounts of HSV-2 DNA in a quantitative PCR test. Overall, we obtained unambiguous...
One or both UL39 ddPCR assays failed for 9% of genital swab DNA specimens, correlating with low amounts of HSV-2 DNA in a quantitative PCR test. Overall, we obtained unambiguous UL39 genotyping data from 152 (91%) of 167 persons on whom it was attempted. Our final data set (Table...
In this embodiment, the HSV-1 glycoprotein D (gD) gene is targeted for amplification and for HSV-2 the target is the glycoprotein G (gG) gene. Unlike “nested” PCR that uses 2 rounds of amplification to achieve the required sensitivity with 2 different primer sets for specificity, real-ti...