17 DNA was extracted and PCR testing was performed using type-common primers to the HSV glycoprotein B gene, with the first positive swab from each participant confirmed to be HSV-1 using a type-specific probe.1
ThePlexPCR®HSV-1&2, VZV kit simultaneously detects and differentiates Herpes simplex virus 1 (HSV-1), Herpes simplex virus 2 (HSV-2) and Varicella zoster virus (VZV) nucleic acids isolated and purified from cutaneous or mucocutaneous lesion swab specimen. ...
p pObjective/p pTo evaluate the utility of a single quantitative PCR (qPCR) measurement of HSV (HSV-12) DNA in cervicovaginal lavage (CVL) specimens collected from women with predominantly chronic HSV-2 infection in assessing genital HSV shedding and the clinical course of genital herpes (GH)...
SI Fig. 1). One or bothUL39ddPCR assays failed for 9% of genital swab DNA specimens, correlating with low amounts of HSV-2 DNA in a quantitative PCR test. Overall, we obtained unambiguousUL39genotyping data from
The PCR with reverse transcription (RT–PCR) was performed using the thermal cycling protocol: RT (incubated 50 °C for 15 min),Taq activation (95 °C for 2 min), followed by 15 cycles of (95 °C at 15 s and 60 °C at 4 min). Pre-amplified cDNA was diluted ...
PCR amplification of both HSV-1 and HSV-2 DNA was performed on a 7500 Fast Real-Time PCR system (ABI) using the following conditions: pre-incubation at 50 °C for 2 min and 95 °C for 10 mins followed by 40 cycles consisting of a denaturation step at 95 °C for 15 ...
Swabs were collected from the genital area, often at the site of a genital lesion, using a Dacron swab, and placed into 1 ml of 1X PCR buffer. Swabs were stored at − 20 °C in Seattle, WA. All participants provided informed consent for the collection of genital swabs and procedures...
17 HSV-1 Detection DNA was extracted and PCR testing was performed using type-common primers to the HSV glycoprotein B gene, with the first positive swab from each participant confirmed to be HSV-1 using a type-specific probe.18 Swabs with at least 2.3 log10 HSV-1 copies/mL (3 copies...
FIG. 1. Real-time PCR using hydrolysis probes. During the DNA amplification process, an amplicon-specific oligonucleotide probe hybridizes to one strand of the amplicon. The probe contains a 5′-reporter fluorochrome (blue) and a 3′-quencher fluorochrome (green) Fluorescence emission from the ...
The PCR with reverse transcription (RT–PCR) was performed using the thermal cycling protocol: RT (incubated 50 °C for 15 min),Taq activation (95 °C for 2 min), followed by 15 cycles of (95 °C at 15 s and 60 °C at 4 min). Pre-amplified cDNA was diluted ...