Protocols were approved by the University of Washington Institutional Animal Care and Use Committee. Vaccine constituents The pVAX1 plasmid (Invitrogen, Carlsbad, CA) was used as negative control. pVAX1-gD21-340 was obtained by PCR-cloning the HSV-2 US6 (gD2) gene, predicted to encode amino ...
HSV-1 Ab titers were expressed as antibody index (AI), calculated by dividing OD measurement generated from the assay by OD cut-off calibrator. Quantitation of the four different HSV-1 IgG subclasses was carried out by a modified ELISA assay (IBL International), using four biotinylated subtype...
(amplicons) are attractive for gene transfer into neurons because they efficiently transduce neurons, have a large capacity (51 kb or 149 kb HSV-1 vectors have been reported (Wade-Martins et al., 2003, Wang et al., 2000)), and can support long-term neuronal-specific, or neuronal subtype...
Latency-associated transcript (LAT) exon 1 controls herpes simplex virus species-specific phenotypes: Reactivation in the guinea pig genital model and neuron subtype-specific latent expression of lat. J. Virol. 2009, 83, 10007–10015. [CrossRef] [PubMed] 48. Bertke, A.S.; Patel, A.; ...
the target tumor tissue without the MOI limitation. The solid tumor targeting feature of BF is depended on the anaerobic microenvironment. Therefore, the application of BF-rTK/GCV is suitable for any kind of solid tumor without receptors or the histological origin and subtype classification of ...