Herpes simplex virus type 2IgG Inhibition assayConfirmatory testingCDC guidelines recommend confirmatory testing of sera with low-positive indices (1.10-3.50) in the HerpeSelect (HSLT) HSV-2 IgG screening assay. To determine if this recommendation is adequate for our patient population, we reviewed ...
Next, the tissue was incubated at room temperature with biotinylated goat anti-rabbit IgG, the avidin-biotin horseradish peroxidase complex and finally AEC substrate. Sections were counter stained with hematoxylin and examined under the light microscope. As a control for the specificity of immuno-...
Among HSV-1 IgG-positive samples, the median HSV-1 IgG index was also significantly higher for the inhibition-indeterminate group (median, 48.8) than for the inhibition-negative (median, 30.7) and inhibition-positive groups (median, 31.2; P = 0.00013 and P = 3.1e - 05, respectively)....
Immunofluorescence of endogenous protein during HSV-1 infection is limited by the presence of the HSV-1 IgG Fc receptor that can bind non-specifically to the primary and/or secondary antibodies used in these assays. In the absence of the HSV-1 glycoprotein gE this complex cannot form [47], ...
Inhibition of multiple-sclerosis-associated retrovirus as biomarker of interferon therapy. J. Neurovirology 2008, 14, 73–77. [Google Scholar] [CrossRef] [PubMed] Rolland, A.; Jouvin-Marche, E.; Viret, C.; Faure, M.; Perron, H.; Marche, P.N. The envelope protein of a human ...
In addition, this work focused on the precise examination of the effect of novel semi-solid vehicles with TA-AgNPs on inhibition of HSV-1 and HSV-2 in both in vitro and in vivo models. 2. Results and Discussion 2.1. Characterization of TA-AgNPs-Based Hydrogels Based on the preceding ...
Interestingly, an analysis of bovine herpesvirus Type I (BHV-1) revealed that TAP inhibition and interaction of gN with gM as well as incorporation of both proteins into the envelope of extracellular virions are independent properties, indicating that gN could perform two different functions, one ...
Further, pharmacologic inhibition or genetic knock-down of HMGB1 reveals a role for HMGB1 in viral restriction, the ability to modulate bystander cell proliferation, and drug sensitivity in 3T6 cells. These data further support the multifactorial role of HMGB1, and suggest it could be a target ...