Herpes simplex virus type 1 (HSV-1) amplicon preparations are usually quantified as transducing units/ml (TU/ml), with little information on genomic copy/TU ratios. In the present study, two HSV-1 amplicons expressing enhanced green fluorescent protein (EGFP) were analysed by quantitative PCR (...
Quantitative Genomic DNA from Human herpesvirus 1 (HSV-1) 规格: 货期: 编号:B214478 品牌:Mingzhoubio 标准菌株 定量菌液 DNA RNA 规格: 冻干粉 斜面 甘油 平板 商品简介 购买须知 联系我们 产品名称Quantitative Genomic DNA from Human herpesvirus 1 (HSV-1) ...
For RNA analysis, the few microglia present in the culture was removed by fluorescence-activated cell sorting (FACS) 500 CD45 À cells in to each well on a 96-well plate and analysed on BioMarker (Fluidigm) or by regular quantitative PCR (qPCR). The astrocytes and the mixed glial cells...
HSV-1 genomes were quantified using quantitative PCR (ABI 7500, Applied Biosystems) using HSV-1 specific primers and Fast SYBR Green Master Mix (Life Technologies). Primers are listed in Supplementary Table 1. Time-lapse fluorescent microscopy Immediately after the addition of HSV-1 in cell ...
实时荧光定量PCR(quantitative real-time PCR,qPCR)是指在PCR反应体系中加入荧光基团,利用荧光信号积累实时监测整个PCR进程,*通过标准曲线对未知模板进行定量分析的方法。实时荧光定量PCR的化学原理包括探针类和非探针类两类,探针类是利用与靶细胞序列特异性杂交的探针来指示扩增产物的增加,非探针类是利用荧光染料或者特异...
(Fig.2D, F). Quantitative RT-PCR results showed that the mRNA levels of these antiviral mediators in WT and TMLeD/LeDmicroglia were comparable (Additional file5: Fig. S3B). We observed reduced antigen-presenting activities of microglia in the brains of infected TMLeD/LeDmice at 5 dpi (...
根 据国内外文献检索,很少有关于GH患者皮疹部 位HsV病毒载量的研究,可能是由于标本取样标 准化及可比性的原因.本研究通过抽取等量疱液 进行FQ—PCR测定的方法,检测分析GH患者皮 损HSV一2病毒载量及其与患者细胞免疫学功能 的相关性,了解患者病毒感染情况及机体整体的 免疫能力,为临床上针对性治疗尤其是一些免疫 ...
实时荧光定量PCR(Quantitative Real-time PCR)是一种在DNA扩增反应中,以荧光化学物质测每次聚合酶链式反应(PCR)循环后产物总量的方法。通过内参或者外参法对待测样品中的特定DNA序列进行定量分析的方法。在PCR扩增过程中,通过荧光信号,对PCR进程进行实时检测。由于在PCR扩增的指数时期,模板的Ct值和该模板的起始拷贝数存...
Similar data were obtained by the real-time quantitative PCR analysis of viral DNA (Fig. 4c) and by the immunofluorescence analysis of HSV-1 infected cells (Fig. 4d). Then, using immunoblot we analyzed the expression of the immediate early protein ICP0, the early protein ICP8, and ...
RNA was extracted by using TRIzol reagent. cDNA was derived from 1 μg total RNA by reverse transcription using Moloney murine leukemia virus reverse transcriptase and oligo(dT) primer. Quantitative PCR was performed by SYBR Premix EX TaqII (Takara) using a CFX96 Real-Time System (Bio-Rad)....