HSV-1 strain McKrae was propagated in green monkey kidney (Vero) cells and maintained at a stock concentration of 1.7 × 108to 1.7 × 109plaque-forming units (PFU)/mL. Anesthetized mice were infected by scarification of the corneal surface with a 25-G 1½” needle and tear ...
Mice were infected using corneal scarification using 5 × 105 PFU of HSV-1 McKrae strain. a–c Tissue plaque assay for trigeminal ganglion (left), brainstem (middle), and brain (right) (n = 5 mice per group). Two-tailed Mann–Whitney U test was used where p < 0.05 ind...
HSV-1 strain McKrae is more neuroinvasive than HSV-1 KOS after corneal or vaginal inoculation in mice. Virus Res. 2013;173:436-440.Wang H, Davido DJ, Morrison LA (2013) HSV-1 strain McKrae is more neuroinvasive than HSV-1 KOS after corneal or vaginal inoculation in mice. Virus Res ...
In mice, BUDR/autoradiography and restriction enzyme analysis identified both HSV-1 McKrae and W strains as having super infected the TG, established latency and reactivated. Also, the avirulent TK negative inoculating strain was altered to a virulent TK positive strain through possible in vivo ...
HSV-1 stocks (McKrae strain, originally obtained from Bryan Gebhardt, LSU Health Sciences Center, New Orleans, LA) were propagated in Vero cells. Stocks were stored at − 80 °C at a concentration of 1 × 10 TLR 9 deficient mice shed more HSV-1 in tears than wild type mice HSV-1 ...
Adenosine-5'-monophosphate (AMP) was evaluated for efficacy in the prevention of spontaneous and induced herpes simplex virus type 1 (HSV-1) ocular shedding in latently infected rabbits with strain McKrae. Intraperitoneal injections (IP) of AMP (100 mg/kg) or NaCl (10 mg/kg) were given on...
The results of showing a reduction in the abundance of specific nuclear speckle components in 17syn+-infected cells prompted us to further investigate the impact of various HSV-1 strains with differing neurovirulence (KOS, 17syn+, McIntyre, and McKrae) on nuclear speckles (Fig. 10). Immunohist...
(Honda,K.,etal.,Nature2005,7434:772-777).Incontrast,thepresentstudydemonstratedthatIRF-3-/-miceare8significantlymoresusceptibletoHSVinfectionviathecornealandintracranialroutes.9Followingcornealinfectionwith2x106pfuofHSV-1strainMcKrae,50%ofwild-type10micesurvived,comparedto10%ofIRF-3-deficientmice....
与DLSPTK不同,DM33的突变基于McKrae毒株,具有更强的增殖能力并诱导更强烈的抗肿瘤效应。在颅内携带U-87MG人神经胶质瘤的裸鼠模型中,小鼠接受DM33治疗后其生存时间延长,组织学分析显示DM33治疗组加速了肿瘤细胞溶解。DM33的安全性在于其对神经元细胞,星形胶质细胞和内皮细胞的减毒作用。 NV1020是衍生自HSV-1的减毒...
DNA previously purified from HSV-1 (McKrae) strain was used as a positive control and DNA previously extracted from an uninfected animal was used as a negative control. HSV-1 specific primers were used to detect HSV-1 (F strain) DNA. The primers and probe detect the HSV-1 gD gene ...