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(Hsp90) is a molecular chaperone that employs the free energy of ATP hydrolysis to control the folding and activation of several client proteins in the eukaryotic cell. To elucidate how the local ATPase reaction in the active site couples to the global conformational dynamics of Hsp90, we ...
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At 9 months of age, mice (N = 6 for each condition) were injected with AAV9 expressing Aha1, FKBP52, or mCherry (control) using a robotic stereotaxic surgery unit (Neurostar GmbH, Tubingen, Germany). A total of 2 µL of 1 × 1012 genomes/mL viral particles was delivered...
Photographs were taken using a confocal microscope (Carl Zeiss Microscopy GmbH, Germany). Western blot After extraction, proteins in cell lysates were first resolved by sodium dodecyl sulfate polyacrylamide gel electrophoresis(SDS-PAGE) and then transferred to nitrocellulose membrane, which was ...
(Takara Biotech, Dalian, China), mRNA isolation kits (Amersham, Uppsala, Sweden), a First Strand cDNA Synthesis Kit (Sangon, Shanghai, China), 20E (Sigma, St Louis, MO, USA), JH III (Sigma, St Louis, MO, USA) and methoprene (a JH analog) (Dr. Ehrenstorfer; GmbH, Augsburg, ...
The results support the potential of using dynamics-based approaches to develop original mechanism-based cancer therapeutics. 2017 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.doi:10.1002/chem.201782262wileyChemistry - A European JournalD'Annessa, I. et al. Design of Allosteric Stimulators of the ...
For comparison of the samples, the intensity of biotin-labeled EF-2 was evaluated via densitometry by using the Adobe Photoshop software (version 7.0, Adobe Systems GmbH, Munich, Germany). Toxin translocation assay with intact cells The pH-dependent translocation of DT across the cytoplasmic ...
Butler, R., Wood, J. D., Landers, J. A. & Cunliffe, V. T. Genetic and chemical modulation of spastin-dependent axon outgrowth in zebrafish embryos indicates a role for impaired microtubule dynamics in hereditary spastic paraplegia.Dis. Model. Mech.3, 743–751 (2010). ...