结论:原阿片碱可抑制HSC-LX2细胞增殖,诱导其凋亡,可降低α-SMA、Collagen Ⅰ、Collagen Ⅲ、TIMP-1表达,升高MMP-2表达有关。 ABSTRACT: OBJECTIVE: To investigate inhibitory effects of protopine on the proliferation of human hepatic stellate cells HSC-LX2 and to explore its mechanism preliminarily. METHODS...
方法:在建立体外过氧化氢 (H 2 O 2 )致 HSC - LX2 细胞氧应激模型基础上,用 MTT 法检测丹皮酚对 HSC - LX2 细胞增殖的影响,生化检测 HSC - LX2 细胞内 MDA 及 SOD,荧光定量 PCR 检测丹皮酚对氧应激致 HSC - LX2 细胞 TGF -β1、Smad4 和 Collagen ImRNA 表达的影响。结果:丹皮... ...
眼镜蛇毒细胞毒素的分离纯化及细胞毒素-1选择性抑制HSC-LX2细胞的研究
Materias and Methods: LX-2 cells (a human hepatic stellate cell) were treated by leptin (positive control), media (control negative), or were transfected by empty plasmid and pcDNA3.1/mda-7. The inflammatory state was evaluated through measuring the mRNA expression level of inflammatory molecule...
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图1 L-02上清液对HSC-LX2细胞α-SMA表达的影响 1:正常组;2:模型组;3:上清原液组;4:1/2上清液组;5:1/4上清液组;与正常组比较:#P<0.05;与模型组比较:*P<0.05, **P<0.01 2.3 对HSC-LX-2细胞α-SMA mRNA的表达 L-02细胞上清液与HSC- LX-2细胞共培养后,上清原液组、1/2上清液组中HSC- LX-2细胞...
结论:1,AngⅡ诱导HSC-LX2氧化应激时可产生两种自由基即羟自由基和单线态氧自由基; 2,不同时间应用中药鳖甲煎口服液复方均可有效抑制AngⅡ诱导HSC-LX2氧化应激时产生的羟自由基,但对单线态氧自由基效果较弱; 3中药鳖甲煎口服液复方在抑制细胞氧自由基的产生,拮抗ACE-Ang Ⅱ-AT1R轴的作用;同时,增强Ang(1-7),...
体外研究选取HSC-LX2细胞株作为本论文体外研究对象.胶原晶格收缩和骨架染色表现HSC收缩;Western Blot和免疫荧光用于检测磷酸化MLC2蛋白水平;葡萄糖摄取,葡萄糖消耗,乳酸生成,细胞外酸化率试剂盒检测糖酵解通量及产物水平;Real-Time PCR和Western Blot用于检测糖酵解限速酶:己糖激酶2,磷酸果糖激酶1和M2型丙酮酸激酶的...
Western Blot examination results were analyzed by using β-actin as internal reference protein.Results The examination results by Real-time RT-PCR showed that after HSC-T6 cells of rats were transfected by pGPU6-3, th...
纯度规格: 1×10(6)cells/T25培养瓶 产品类别: 实验试剂 "HSC-4细胞:人口腔鳞癌细胞 细胞物种来源:人源或鼠源等其它物种来源 HSC-4 Cell 细胞形态特性:上皮细胞 16461-94-2 实验室细胞培养基知识简介:干粉培养基:以前大部分实验室都是用干粉培养基,但配制过程就较为繁琐,要溶解、调pH值,过滤,过程中可能...