HPV检测的敏感性和特异性(91.3%, 50.0%)与hsa-miR-124-2基因甲基化的敏感性和特异性(86.7% and 61.3%)相比差异没有统计学意义。 经多元回归分析,诊断高级别病变的独立预测因子在高危型HPV检测中也显示出阳性结果(OR = 5.5; 95% CI = 2.6 to11.6) ,包括以下基因:hsa-miR-124-2 (OR = 5.1; 95% CI ...
目的观察hsa-miR-124-3p.1通过靶向肿瘤坏死因子受体相关因子6(TRAF6)抑制转化生长因子β1(TGF-β1)对人胃癌细胞上皮间质转化,迁移和侵袭的影响.方法收集胃癌组织和癌旁正常组织各43例,培养人胃黏膜上皮细胞GES-1及胃癌细胞NCI-N87,MGC-803,BGC-823,SGC-7901,MKN-45,采用荧光定量PCR技术和蛋白印迹法检测组织...
in plasma by hsa-miR-124a-3p RT-qPCR assay. All test results were statistically analyzed to evaluate the clinical values between RT-qPCR assay and Down’s screening。 Results: 1. The reverse transcription primer of hsa-miR-124a-3p for RT-qPCR testing was designed and synthetized and...
hsa-miR-124-3plncRNAmiRNAAcute lymphoblastic leukemia (ALL) is one of the deadly forms of childhood cancers in the world. In the present study, we used both in vitro and in vivo models to evaluate the functional mechanisms of a long noncoding RNA (lncRNA), small nucleolar RNA host gene...
这条通路会保持激活状态,并在宫颈高级别病变的发展过程中发挥作用。 特定基因启动子区甲基化,如hsa-miR-124-2,SOX-1, TERT, and LMX1A可以作为检测宫颈癌前病变的辅助手段,而且这项客观的检测方法可以利用宫颈细胞学和HPV DNA检测的样本。将甲基化谱分析与细胞学和HPV DNA联合更具预测性。
目的通过构建不同hsa-miR-124-3p表达载体后对其进行靶点验证分析,研究hsa-miR-124-3p调控CD151蛋白表达的过程,探讨hsa-miR-124-3p在胃癌发生与发展中的重要作用.方法利用脂质体载体转染技术对MGC803胃癌细胞株进行转染,建立高,中(对照组),低3组不同hsa-miR-124-3p表达量MGC803细胞模型,通过实时荧光定量PCR检测...
Among these, hsa-miR-124-3p shows significant potential as a prognostic marker for therapeutic efficacy in athletes, providing a new avenue for early detection and management of multiple myeloma in this specific population.Hui GongChunxiang Kang...
Hsa-mir-124-3 CpG island methylation is associated with advanced tumours and disease recurrence of patients with clear cell renal cell carcinoma. Gebauer K,Peters I,Dubrowinskaja N, et al. British Journal of Cancer . 2013Gebauer K, Peters I, Dubrowinskaja N, Hennenlotter J, Abbas M, ...
Among them, hsa‐mir‐34a seems to be significantly upregulated, while hsa‐mir‐124 and hsa‐mir‐204 are significantly downregulated. The target genes of the signature miRNAs are mainly enriched in hsa04015:Rap1 signaling pathway, hsa04142:lysosome, and hsa04360:axon guidance, indicating that ...
D. (2010). Methylation-mediated silencing and tumour suppressive function of hsa-miR-124 in cervical cancer. Mol Cancer 9, 167.Wilting SM, van Boerdon k R A, Hen ken F E, et al: Methylation‑mediated silencing and tumour suppressive function of hsa‑miR‑124 in cervical cancer. Mol...