1.4 qRT-PCR检测血清hsa-miR-22-3p、hsa-miR-103a-3p水平 于清晨空腹抽血5 mL,收集上层血清,采用Trizol试剂盒提取血清中总RNA,经逆转率为cDNA模板,采用实时荧光定量PCR仪扩增血清中hsa-miR-22-3p、hsa-miR-103a-3p。程序设置条件为:95 ℃预变性90 s,95 ℃变性30 s,58 ℃退火30 s,72 ℃延伸20 s,共...
hsa_circ_0062389 promotes the progression of non-small cell lung cancer by sponging miR-103a-3p to mediate CCNE1 expression.doi:10.1016/j.cancergen.2019.12.004Yahui SheYuanyuan HanGuangting ZhouFangyan JiaTan YangZuojun ShenCancer Genet
目的:探讨hsa-miR-103a-3p影响食管鳞状细胞癌(esophageal squamous cell carcinoma,ESCC)细胞对奥沙利铂(oxaliplatin,OXA)耐药的机制.方法:采用RT-qPCR验证测序结果中hsa-miR-103a-3p在不同ESCC细胞系中的表达;转染hsa-miR-103a-3p mimics或inhibitor,验证hsa-miR-103a-3p是否可以逆转ESCC细胞系的耐药表型.应用...
miR-103a-3pCCNE1Non-small cell lung cancerRecently, increasing evidence showed that circular RNAs (circRNAs) play critical roles in tumor progression. However, the roles of hsa_circ_0062389 in non-small cell lung cancer (NSCLC) development remain unclear. In the present study, hsa_circ_0062389...
Conclusion CircBAGE2 contributes to PCa progression by upregulating CCND1 and PDCD10 expression through its role as a 'sponge' of miR-103a-3p. CircBAGE2 may be a potential therapeutic target for PCa.doi:10.1007/s10330-020-0454-4Chunlei ZhangDan LiuQinqin TianQi YangXiaoyuan ZiYinghao Sun《...