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每孔按10 μg进行上样,经10%十二烷基硫酸钠(sodium dodecylsulphate,SDS)-聚偏二氟乙烯(polyvinylidene fluoride,PVDF)恒压80 V电泳分离30 min、120 V分离40 min;冰水浴恒流240 mA转膜;脱色摇床上用10%脱脂奶粉封闭2 h;4℃摇床温...
早期非小细胞肺癌患者hsa—mir-34b基因启动子甲基化水平检测
The miR-12528 expression was aberrantly downregulated in cancer cell lines and in the patient tissues derived from human non-small cell lung cancer. In addition, we found that miR-12528 post-transcriptionally controls the translation of the insulin-like growth factor 1 receptor (IGF-1R) gene by...
Among them, AC005154.6/hsa- miR-29c-3p, hsa-miR-29b-3p, and hsa-miR-29a-3p/ CCNL2 showed the highest correlation between mRNA and lncRNA (coefficient of correlation is 0.8) (Additional file 1: Figure S4) and have not previously been reported in PRAD. Based on this, we selected...
2.5.1. Quantitative real-time PCR of miRNA expression To analyze cellular uptake of ExomiR-365a-3p by the HepG2 cell line, cells were seeded in 6-well plates (5 × 105 cells per well) and allowed to adhere for 24 h. Then, the cells were treated with 14 μg/ml of ExomiR-365a...
Mechanically, we found that hsa_circ_000984 may act as a competing endogenous RNA (ceRNA) by competitively binding miR-106b and effectively upregulate the expression ofCDK6, thereby inducing a series of malignant phenotypes of tumor cells. Taken together, these observations suggest that the hsa_...
miR-122-5p and -885-5p were up-regulated in individuals with FL (fold change (FC) = 1.55, p = 1.36 * 10−14and FC = 1.25, p = 4.86 * 10−4, respectively). In regression model adjusted with age, sex and BMI, hsa-miR-122-5p and -885-5p ...
miR-29b和骨糖蛋白验证结果显示,circ_0076906、miR-29b及骨糖蛋白基因在疏松症患者和对照组骨组织和血清中均呈差异表达,circ_0076906可诱导成骨分化,其沉默可抑制人间充质干细胞的成骨相关基因(骨钙素和RUNX2)表达,circ_0076906可充当miR-29b调节的骨糖蛋白基因表达的海绵;⑤上述数据说明,实验成功构建了骨质疏松...
本发明提供一种小分子非编码RNA(该小分子非编码RNA命名为hsa-miR-29b)及其应用.本发明进行了体外管形成实验和裸鼠成瘤及免疫组化实验,体外管形成实验的结果表明体外恢复hsa-miR-29b表达可显著抑制肿瘤细胞诱导的血管生成;裸鼠成瘤及免疫组化实验的结果表明体内恢复hsa-miR-29b表达可显著抑制肿瘤细胞的体内成瘤能力和...