本发明涉及一种用于胃癌诊断的环状RNA分子标记物,其特征在于:该环状RNA为hsa_circ_0001017,本发明还提供一种检测血浆中状RNA分子标记物的方法,包括如下步骤:(1)采集血液,提取血浆中的总RNA;(2)将总RNA逆转录成cDNA;(3)利用特异性扩增背对背引物和看家基因GAPDH的扩增上下游引物对步骤(2)的cDNA溶液进行数字微滴...
摘要: 本发明涉及一种用于胃癌诊断的环状RNA分子标记物,其特征在于:该环状RNA为hsa_circ_0001017,本发明还提供一种检测血浆中状RNA分子标记物的方法,包括如下步骤:(1)采集血液,提取血浆中的总RNA;(2)将总RNA逆转录成cDNA;(3)利用特异性扩增背对背引物和看家基因GAPDH的扩增上下游引物对步骤...查看全部>> ...
Circ_0001017 may downregulate miR-145-5p through direct binding. Furthermore, the expression of miR-145-5p was negatively regulated by circ_0001017 in OS cells. In addition, further functional studies indicated that miR-145-5p inhibitor eliminated the effects caused by si-circ_0001017 in OS ...
Hsa_circ_0001017Competing endogenous RNAmiR-197Ras homolog family member BBackground Gastric cancer (GC) is one of the most common digestive system diseases and yet lacks effective therapeutic regimen. Aims The aim of our present research was to probe the value of hsa_circ_0001017 in GC ...
Background Gastric cancer (GC) is one of the most common digestive system diseases and yet lacks effective therapeutic regimen.AimsThe aim of our present research was to probe the value of hsa_circ_0001017 in GC treatment.MethodsqRT-PCR and Western blot were performed to detect gene and ...