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And aren’t the trains to Milton Keynes less full because they no longer have Birmingham and Manchester passengers on them (ie, you are looking at capacity in terms of numbers of paths not numbers of passengers – or even the number of trains per hour at any given station – for example,...
4 Hybridization, Capture, and Amplification This section describes the steps to hybridize and capture targeted fragments in the prepared DNA library using a SureSelect Probe, using fast (2-3 hour) hybridization conditions and standard SureSelect XT HS2 reagents. Post-capture library amplification ...
Add another 5 minutes per hour’s journey for other sectors if you want to, as a rule of thumb. You still don’t need more than 9 basic HS platforms if 18 tph max – HS2 has confirmed to me an average of 2 trains per platform per hour is their plan – plus 2 extra as perturba...
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In order to have enough reagent to run 3 full columns (i.e., 24 wells), you must purchase 4 units of the 6-Hyb Probe. The 12-Hyb Probes support target enrichment for 96 samples pooled using the configuration recommended for the included designs of 8 samples/pool (for 12 hybs × 8...
NOTE If performing same-day hybridization and capture, begin the bead preparation steps below approximately one hour after starting hybridization in step 8 on page 53. If performing next-day capture after an overnight hold at 21°C, begin the bead preparation steps below on day 2, just before...