HRP常用的化学发光底物为鲁米诺(3-氨基苯二甲酰肼)、异鲁米诺(4-氨基苯二甲酰肼)及其衍生物。用于辣根过氧化物酶(HRP)的化学发光底物多为双组分系统,稳定的H2O2和鲁米诺(5-氨基-邻苯二甲酰肼)是经典的HRP标记CLEIA反应体系。 1)HRP-H2O2-鲁米诺发光体系 鲁米诺(Luminol)又名发光氨,是一种化学荧光分子。碱性...
目的:合成和表征N-酚噻嗪丙磺酸,探讨其对HRP-Luminol-H2O2化学发光体系的增强作用.方法:将N-酚噻嗪丙磺酸加入HRP-Luminol-H2O2化学发光体系,记录发光强度和发光持续时间,并用于HRP含量的测定.结果:N-酚噻嗪丙磺酸可使HRP-Luminol-H2O2化学发光体系的发光强度增强,发光持续时间达30分钟以上.HRP含量在25-800 pg/mL...
玩转ELISA | 化学发光试剂盒实验操作视频详解 化学发光试剂盒(CLIA kit)采用 Streptavidin-HRP 及增强型的 ECL 发光体系作为发光底物试剂来实现检测过程的试剂盒。将代测样本加入到固相包被有抗体的黑色不透明微孔板中,辣根过氧化物酶的结合物和抗体特异性结合。洗去未反应的游离成分。加入鲁米诺(Luminol)发光底液,并...
evaluation of the antioxidant activity of liposomal flavonols by the HRP–HO–luminol systemAna Paula LandiLibrandiCarlos Alberto de OliveiraLuciana Mariko KabeyaJosé Orestes Del CiampoYara Maria LucisanoValim
A luminol-H2O2-HRP chemiluminescence system with high relative luminescent intensity (RLU) and long stabilization time was investigated. First, the comparative study on the enhancement effect of ten compounds as enhancers to the luminol-H2O2-HRP chemiluminescence system was carried out, and the results...
本发明涉及一种免疫检测方法,特点是一种经免疫层析后在层析膜特定的区域形成抗原-抗体复合物区带,以含有Luminol的溶液催化免疫复合物区带上的辣根过氧化物酶(HRP)发光,用X线胶片自显影或光吸收仪器判读实验结果,以提高免疫层析法检测的灵敏度,可进行定性或定量分析.用于抗原,半抗原和抗体的检测.李川江...
用Luminol作为底物催化HRP发光的免疫层析方法及其检测试纸条 本发明涉及一种免疫检测方法,特点是一种经免疫层析后在层析膜特定的区域形成抗原—抗体复合物区带,以含有Luminol(5’-氨基-2,3-二氢-1,4-酞嗪二酮)的溶液催化免疫复合物区带上的HRP发光,并用X线胶片自显影或光吸收仪器检测。 免疫层析法作为免疫分析...
In addition, the amount of HRP used in the assay with enhancers is 40 times less than in the assay without them. The study of the specificity of the developed assays demonstrated that in the assay without enhancers several phenol compounds, for instance, unsubstituted phenol, suppressed the CL...
The present invention relates to a luminol - hydrogen peroxide - the HRP method -HRP-BSA bromophenol red chemiluminescence detection system. 目的是建立新型的化学发光体系,将其用于高灵敏度检测HRP. The aim is to establish a new chemiluminescence system, which was used in high-sensitivity detection of...
Three formulations of the Luminol-H2O2-HRP-enhancer CL system.Yang, LihuaJin, MaojunDu, PengfeiChen, GeZhang, ChanWang, JianJin, FenShao, HuaShe, YongxinWang, Shanshan