The sample was then analyzed by HPLC-FLD-MS and quantitated by addition of an external standard. This method was compared analysis of GSL glycans after cleavage by an Endoglycoceramidase (EGCase) enzyme and labeling with a fluorophore (2-anthranilic acid, 2AA). The two methods are ...
论文标题: 柱前衍生HPLC/FLD-APCI/MS法同时测定藏药悬钩木中6种三萜酸类成分的含量 英文标题: 中文摘要: 目的:建立同时测定藏药悬钩木中山楂酸、科罗索酸、桦木酸、路路通酸、齐墩果酸、熊果酸等6种三萜酸类成分含量的方法。方法:采用柱前衍生高效液相色谱-荧光检测/质谱联用法。选择2-(7H-二苯并[a,g]咔...
结果表明,两种方法平均回收率均大于70%,相对标准偏差均小于10%,检出限分别为0.0001μg/L和0.0003μg/L,均能满足测定要求,测定低浓度苯并(a)芘样品时,高效液相色谱法更稳定,峰形基线平稳,检出限低,回收率高.净化和未净化的苯并(a)芘在用GC-MS和HPLC-FLD两种方法定量计算浓度时,无显著性差异.在分析基质复杂...
fld是氢火焰检测器？？？荧光检测器跟质谱比，最近看见些文章，hplc后面串这两种设备的检测限都一个数量...
fld跟ms相比，检测限或定量限可以相差多少倍？
SensitivedeterminationofmonoterpenealcoholsinurinebyHPLC–FLD combinedwithESI-MSdetectionafteronline-solidphaseextractionofthe monoterpene-coumarincarbamatederivates RolandJ.W.Meesters a,b,∗ ,MikeDuisken b,d ,HeikeJähnigen b ,JulianeHollender b,c a UniversityofArkansasforMedicalSciences(UAMS),Centerfor...
Composition of tocochromanols was studied in seeds recovered from eight pear (Pyrus communis L.) cultivars, six dessert and two interstock, grown in a single environment. Four tocopherol (T) homologues, namely α, β, γ and δ, were quantified in all tested samples by RP-HPLC/FLD method...
GC/MSHPLC针对黄酒特性,采用固相萃取-气相色谱/质谱联用法(SPE-GC/MS)和高效液相色谱-荧光法(HPLC-FLD)分别测定黄酒中氨基甲酸乙酯(EC)含量,并进行了系统优化和比对.结果表明,SPE-GC/MS和HPLC-FLD法均能准确检测黄酒中的EC含量,测定结果可靠.其中,SPE-GC/MS法灵敏度较高,已被国际权威机构AOAC,OIV和欧盟纳入...
与国标相比,本研究的HPLC-FLD法采用梯度洗脱程序.GC-MS法色谱条件为:HP-5MS毛细管柱(30 m×0.25 mm×0.25μm),梯度升温程序,进样口和传输线温度280℃,脉冲不分流进样,载气流速1.2m L/min.MS条件:EI离子源,离子源230℃,四级杆150℃,选择离子模式(SIM)定量.线性方程分别为Y=817883X+39074(R2=0.9999)和Y...
与国标相比,本研究的HPLC-FLD法采用梯度洗脱程序.GC-MS法色谱条件为:HP-5MS毛细管柱(30m × 0.25mm × 0.25 μm),梯度升温程序,进样口和传输线温度280℃,脉冲不分流进样,载气流速1.2mL/min.MS条件:EI离子源,离子源230℃,四级杆150℃,选择离子模式(SIM)定量.线性方程分别为Y=817883X+39074(R2=0.9999)和...