DPPH-HPLC-QTOF-MS/MS was developed for rapid screening and identification of antioxidants. After reaction with DPPH radical, HPLC peak of thirteen antioxidants in Clerodendrum bungei extract disappeared. By analysis of their retention times, characteristic ultraviol...
建立DPPH-HPLC-QTOF-MS/MS方法快速筛选,鉴定和测定杜仲黑茶中抗氧化活性成分.杜仲黑茶提取物与DPPH自由基反应30min后,9个抗氧化活性成分的HPLC峰消失或峰面积明显减少,结合QTOF-MS/MS谱图及与标准品对照,确定抗氧化活性成分为:没食子酸,新绿原酸,原儿茶酸,绿原酸,咖啡酸,表没食子儿茶素没食子酸酯,槲皮素-3-O...
2010. On-line HPLC-MS-DPPH assay for the analysis of phenolic antioxidant compounds in fruit wine: Antidesma thwitesianum Muell. Food Chem., 118(1), 147-152.Nuengchamnong, N., Ingkaninan, K., 2010. On-line HPLC-MS-DPPH assay for the analysis of phenolic antioxidant compounds in fruit...
通过DPPH自由基清除实验对臭牡丹水提物的抗氧化活性进行评估,并建立DPPH-HPLC-QTOF-MS/MS方法快速筛选和鉴定臭牡丹中抗氧化活性成分.臭牡丹水提物与DPPH自由基反应30min后,13个主要抗氧化活性成分的HPLC峰消失,根据抗氧化活性成分的保留时间,紫外吸收特征光谱,质谱裂解规律和诊断离子,以及与标准品对照,初步确定了13...
目的:运用HPLC-Q-TOF MS/MS偶联1,1-二苯基-2-苦基肼(1,1-diphenyl-2-picrylhydrazyl,DP-PH)添加法对6个甘草黄酮类化合物的肝微粒体代谢产物进行抗氧化活性筛选.方法:将肝微粒体与6个化合物共孵育,经HPLC-Q-TOF MS/MS鉴定代谢产物;孵育样品中加入DPPH,通过比较前后代谢产物峰面积的变化,判断不同代谢产物...
目的 建立基于离线二维色谱-二苯基三硝基苯肼-电喷雾飞行时间质谱结合技术(2D-HPLC-DPPH-ESI-Q-TOF/MS)的金银花中抗氧化成分系统筛选方法.方法 采用离线二维色谱对金银花提取物进行分段富集,分析,第一维采用semi-PREP-HPLC技术对金银花提取物进行极性分段制备和富集浓缩,第二维分别采用亲水和反相色谱技术进行分析;...
HPLC coupled on-line to ESI-MS and a DPPH-based assay for the rapid identification of anti-oxidants in Butea superba. Phytochem. Anal. 2005, 16, 422-428.Nuengchamnong N,de Jong CF,Bruyneel B. HPLC coupled on-line to ESI-MS and a DPPH-based assay for the rapid identification of anti...
CollegeJiaweiCollegeChenCollegeXiasenCollegeJiangCollegeFuLiangCollegeHuCollegeWileyJournal of Food ScienceC. Zhang, X. Shen, J. Chen, X. Jiang, and F. Hu, "Identifica- tion of free radical scavengers from Brazilian green propolis using off-line HPLC-DPPH assay and LC-MS," Journal of Food ...
Rapid and simple method for screening of natural antioxidants from Chinese herb Flos Lonicerae Japonicae by DPPH-HPLC-DAD-TOF/MS . Journal of Separation Science 2008 ; 31 : 3519 – 3526 .Tang D , Li HJ , Chen J , Guo CW , Li P . 2008 . Rapid and simple method for screening of ...
HPLC-DPPH was used to screen out the antioxidant active components in different extracts of fig waste leaves. In order to further study the antioxidant chemical components, HPLC-QTOF-MS/MS-DPPH was applied to identify and analyze the antioxidant active components for the first time. By this meth...