This method enables highly sensitive simultaneous analysis of the 18 abovementioned types of GAG-derived disaccharides using HPLC with fluorescence detection in small amounts of urine (1 mL) in a single run. The versatile method described here could be applied to the analysis of GAGs in other ...
response [25,26]. Response surface plots are useful for establishing the response values and operation conditions as required. Additionally, they can provide a method to visualize the results and help in processing the experimental levels of each variable and the type of interactions between [27]....