(3)Concentration of salts in the buffer component of a mobile phase流动相中缓冲液盐的浓度:不允许调节。 (4)Flow rate流速:当柱子的内径改变时,调节是可以接受的,具体见流速公式。 (5)Column parameters柱参数 Stationary phase固定相:固定相的属性...
液相色谱固定相stationary phase of HPLC二、液相色谱流动相二、液相色谱流动相 mobile phase of HPLC第三节第三节液相色谱的固定相与液相色谱的固定相与流动相流动相high performance liquid chromatographstationary phase and mobile phase of HPLC2022-3-192一、液相色谱固定相一、液相色谱固定相 stationary phases ...
(3)Concentration ofsalts in the buffer component of a mobile phase:用于配制流动相的含水缓冲液的盐的浓度可在±10%范围内调节。 (4)Flow rate:一般调节范围为±50%,若柱尺寸改变了,流速可以更大范围内调节,具体可按下述公式来计算。 (5)Column parameters: Stationary phase:固定相的属性不能改变(例如:不...
The choice of stationary phase, mobile phase, and detection method all play crucial roles in the successful application of HPLC for peptide purity determination.总的来说,HPLC是一种强大而多功能的技术,可用于确定肽的纯度,从而能够在样品中分离、鉴定和定量化不同的肽组分。固定相、流动相和检测方法的...
The influence of the mobile phase pH and the stationary phase type on the selectivity tuning in high performance liquid chromatography nucleosides separation[J] . SylwiaKowalska,KatarzynaKrupczyńska.J. Sep. Science . 2005 (13)Kowalska, S.; Krupczyn´ska, K.; Buszewski, B. The influence of...
(3)Concentration of salts in the buffer component of a mobile phase流动相中缓冲液盐的浓度:不允许调节。 (4)Flow rate流速:当柱子的内径改变时,调节是可以接受的,具体见流速公式。 (5)Column parameters柱参数 Stationary phase固定相:固定相的属性不能改变(例如:不能用C8来代替C18); ...
Incapableofseparatingthepolymerswithacontinuousmol.Wtdistribution,butcapableofdeterminingmolecularweightdistribution ●StationaryandMobilePhasesofHPLC Self-studyProblems WhatkindsofstationaryphasesdoeachHPLCmethodhave?Whatcharacteristicsdoeachstationaryphasehave?HowtoselectmobilephaseinHPLC?
The mobile phase carries a liquid sample through the column to the detector, and compounds or analytes separate due to varying degrees of interaction with the stationary phase. A detector measures the analytes after elution from the column, and a chromatography data system (CDS)...
LAAQ-B-LC001B Stationary Phase and Mobile Phase Used in Normal Phase Mode ? Stationary Phase Cyano type: -Si-CH2CH2CH2CN ? Amino type: -Si-CH2CH2CH2NH2 ? Diol type: -Si-CH2CH2CH2OCH(OH)-CH2OH ? ? Mobile Phase Basic solvents: Aliphatic hydrocarbons, aromatic hydrocarbons, etc. ?
The influences of mobile-phase composition, stationary-phase substrate, and metal on solute retention behavior were examined for the metal-dipivaloylmethane additive systems.doi:10.1080/10408349708050579Lochmüller, C. HHangac, Helen HTaylor & Francis GroupCritical Reviews in Analytical Chemistry...