To make a Run, first be sure the sample you are to run has been FILTERED.为了使运行,首先要确保您正在运行的样品已经被过滤。The procedure below includes a filtration step using Whatman #42 filter paper.下面的过程,包括使用的Whatman#42滤纸过滤步骤。Other procedures may call for forcing the sample...
Trace enrichment and multidimensional liquid chromatography are the key-points which are prerequisites to scale down the sample size for the cleanup procedure. Reduction in scale on one hand and transfer of the individual cleanup steps, e.g. filtration, liquid-liquid partitioning, concentration, ...
Decrease analyte, solvent, or matrix concentration in sample Preventing column/detector overloading, reducing sample solvent elution strength Centrifugation Sedimentation based on density Removing large cellular components from solution Filtration Remove particulate...
To make a Run, first be sure the sample you are to run has been FILTERED.为了使运行,首先要确保您正在运行的样品已经被过滤。 The procedure below includes a filtration step using Whatman #42 filter paper.下面的过程,包括使用的Whatman#42滤纸过滤步骤。 Other procedures may call for forcing the ...
Water is either obtained as HPLC-grade (BDH, Poole, UK; J. T. Baker, Phillipsburg, NJ; or EMD Chemical, Gibbstown, NJ) or purified by an E-pure water filtration device from Barnstead/Thermolyne (Dubuque, IA). 2. Reagent grade ortho-phosphoric acid (H\(_{3}\)PO\(_{4})\)is ob...
The procedure consists of filtration of the dissolution sample and direct injection of the sample onto the HPLC column. Although levothyroxine sodium is present at low concentrations (20鈥 50 ng/ml) in the dissolution samples, its detection and quantitation was possible due to the large volumes ...
However, the plasma filtration using the CF-50 Amicon membranes is a quicker procedure (10 min) also allowing the simultaneous evaluation of acid-labile amino acids such as asparagine. glutamine and tryptophan and a higher recovery of taurine (>96%). The taurine levels in human plasma samples ...
SPE Cartridges Sample Filtration Membrane sample filters are used to clari- fy any samples that need further analysis or use where particulate matter may cause a problem. They are most often used for the filtration of HPLC samples to prevent damage to the injection valve or to the HPLC column...
Several sample preparation techniques such as hydrolysis [7], filtration/dilution [8], liquid extraction [9], ultrasound-assisted extraction [10] and solid phase extraction using molecularly imprinted polymers [11] were developed to allow HPLC-based determination. Reverse-phase high-performance liquid ...
5982-1010 5982-0101 77 SAMPLE PREPARATION Captiva Filtration Filtering samples before analysis can deliver longer column lifetime, less downtime, and optimal instrument performance, because even small amounts of particulate can clog your column inlet or contaminate your system. You can remove damaging...