Preparative LC separations are for the large-scale purification of mass components and often utilized for manufacturing purposes. Columns usually range from 50-200 mm inner diameter and are run at flow rates of > 50 mL/min. Semi-preparative LC applications range from small-scale...
It has been over 3 years since the first publication of the polymer microfluidic HPLC-Chip technology and more than 1 year since this technology became commercially available. Here, we summarize the design principle and fabrication processes of the Agilent HPLC-Chip and review the applications ...
Chapter5-HPLC Chapter5HighPerformanceLiquidChromatography Objectives:1.GraspthecharactersandtheapplicationfieldofHPLC;2.Graspthetheoryofvariousseparationmodesandtheirsuitableanalyticalobjectives,andlearnhowtoselecttheseparationmode.3.Grasptheprinciple,theadvantageanddisadvantage,andtheapplicationfieldofthe...
Exclusion principle assumes no adsorption of polymer molecules on packing material Every polymer will be eluted between Vo and Vt Detectors in Polymer Chromatography Concentration –Response ~ concentration, (C) –Refractometer; ∆N = (dn/dc) C Structure-selective –UV/Vis Detector (Also can use...
They work on the principle that many microorganisms have their own unique mass spectral signature based on the particular proteins and peptides that are present in the cells. Identification of unknown peaks in gas chromatography (GC-MS)-based discovery metabolomics is challenging, and remains ...
The HPLC methodology applied to the analysis of biological samples makes it possible for the identification of many metabolites. Samples from two human embryos culture medium were analysed byhigh-pressure liquid chromatography–mass spectrometry(HPLC–MS). They work on the principle that many microorgani...
LAAQ-B-LC001B Separation improvement due to the principle of hydrophobic interaction LAAQ-B-LC001B Section 10 Normal Phase (Partition) Chromatography ? Partition chromatography in which the stationary phase has a high polarity (hydrophilic) and the mobile phase has a low polarity (hydrophobic) ...
In principle, the type of coating can be varied, like packed HPLC columns. However, in µPACs, the phase ratio is about 0.04. Therefore, solute retention on µPAC with the same solvent composition on a nanoflow HPLC column will be significantly less than on a particle-packed HPLC ...
Principle of Separation in HPLC Preparative HPLC Overview of HPLC Detectors Analysis results About Resolution, Part 1 About Resolution, Part 2 Formula for Calculating the Number of Theoretical Plates Theoretical Plate Number and Symmetry Factor Confirming Peak Integration About the SI System Essentia...
“With Agilent CrossLab, our customers can simplify product selections and purchase, streamline their workday, and improve their productivity,” said Michael Feeney, vice president and general manager, Agilent Supplies Division. “The new HPLC offerings, combined with our existing CrossLab G...