等度分析:按“direct”设定A、B、C、D流动相的百分比,直接输入“flow rate”(0.8-1.5ml/min),按Enter 即可。 梯度分析:按“program method”, 编辑梯度洗脱的程序,按“save”,保存到“program table”(1-15)中,按“operate method”, 输入你在“program table”中所保留的数字(1-15),再按“operate method...
This flow rate will enable all components to elute within 5 min, but is slow enough to ensure resolution of individual peaks. Next, verify the minimum and maximum pressures on the solvent delivery system. These settings shut the pump off in case of a leak or clog, respectively. Press “...
Low-flow columns—also known as nano, capillary, or micro columns—have a smaller diameter compared to analytical columns. Thermo Scientific nano-, capillary-, and micro-flow columns offer excellent sensitivity and resolution in easy-to-use formats: Thermo Scientific EASY-Spray...
An HPLC-EC-method is proposed for the determination of phenol, alkylphenols, hydroxyphenols and chlorophenols. With the application of programmed flowrate variation good resolution within short analysis times could be reached; the eluent is usable for several times. An electrochemical dual-detector ...
Ultra-high-performance liquid chromatography(UHPLC) methods are based on <2 µm stationary phase particles and run at operating pressures between 600-1200 bar withflow rates from 0.2 – 0.7 mL/min. This increased pressure range offers better resolution and sensitivity, higher t...
Low-flow columns—also known as nano, capillary, or micro columns—have a smaller diameter compared to analytical columns. Thermo Scientific nano-, capillary-, and micro-flow columns offer excellent sensitivity and resolution in easy-to-use formats: Thermo Scientific EASY-Spray HPLC Columns Thermo ...
Using the increased separation speed and resolution of the Vanquish UHPLC system, the Thermo Scientific Vanquish Charged Aerosol Detector detects and quantifies non- or semi-volatile substances with near-universal detection capabilities. Integrating into any LC, HPLC, or UHPLC system from any manufact...
Thermo Scientific nano-, capillary-, and micro-flow columns offer excellent sensitivity and resolution in easy-to-use formats: Thermo Scientific EASY-Spray HPLC Columns Thermo Scientific single and double nanoViper UHPLC Columns Thermo Scientific μPAC Neo HPLC Columns ...
HPLC is a very common method for metabolomics analysis. With the invention of electrospray ionization, HPLC is coupled tomass spectroscopy. HPLC has lower chromatographic resolution, requires no derivation for polar molecules and separates molecules in the liquid phase. HPLC has the advantage of much...
Full MS/dd-MS 2 detection mode was adopted ,the resolution of Full MS was 70 000 and the resolution of dd-MS2 was 17 500. The scanning range was m/z 110-1 200. The ion peaks were identified by comparing with the information of control substances ,literature references and self-built ...