C.7.2 Eluent Degassing Methods You can degas eluents using any of the following methods: • In-line degassing • Heating • Vacuum sonication C 93 CC Vacuum Degassing An in-line vacuum degasser operates on the principle of Henry's Law to remove dissolved gases from the solvent. According...
Chapter5HighPerformanceLiquidChromatography Objectives:1.GraspthecharactersandtheapplicationfieldofHPLC;2.Graspthetheoryofvariousseparationmodesandtheirsuitableanalyticalobjectives,andlearnhowtoselecttheseparationmode.3.Grasptheprinciple,theadvantageanddisadvantage,andtheapplicationfieldofthedetectors.了4.Know...
In relation to GC–MS assay for analysis of plasma HPPTCA16, it has been assumed that the procedure involves an external sample (pre)treatment with a reduced number of steps (principle 1); the volume of plasma is 250 µL (principle 2); the analytical device is positioned off-line (...
3.1.4. Selecting a flow rate To enhance the resolution of the eluted peaks, the effect of flow rate was investigated. The flow rate was varied between 0.5 and 2.0 ml/min. A flow rate of 1.0 ml/min was found to be the best for achieving good separation in a reasonable timeTable 1 Ta...
The analytical principle of pattern recognition in chemometrics is usually applied during metabolomics technology analysis. Appropriate chemometrics tools can expedite the analysis process and streamline rapid data processing [17]. Commonly used multivariate statistical analysis methods include principal component...