Tips on Troubleshooting HPLC Systems 1. Initial checks Before understanding the trouble shoot issues, you must first perform all the basic checks to first ensure that the system is correctly set up or not. You must verify that all connections to the system are secure, solvent levels are adequat...
Leaks at detector Flow cell damaged – Replace flow cell. Flow cell blocked – Flush flow cell with solvent. Loose fittings – Tighten all fittings gently. Get In Touch If you have any questions regarding liquid chromatography troubleshooting, get in touch.PREVIOUS...
Application Brief: EASY-Spray Troubleshooting Application Brief: EASY-Spray Best Practices Technical Guide: Low-flow UHPLC-MS Columns Connection Guide Technical Notes Technical Note: μPAC HPLC Column robustness inbottom-up proteomics Technical Note: Routine proteome analysis using 5...
Troubleshooting 13 1.5 Specifications Table 1-3 Operational Specifications Item Specification Chemical resistance Unaffected by full range of organic solvents and aqueous solutions of acids, bases, salts, and surfactants pH range 0 to 14 Gas removal efficiency Varies with flow rate (see Table 1-2) ...
Application Brief: EASY-Spray Troubleshooting Application Brief: EASY-Spray Best Practices Technical Guide: Low-flow UHPLC-MS Columns Connection Guide Technical Notes Technical Note: μPAC HPLC Column robustness inbottom-up proteomics Technical Note: Routine proteome analysis using 50 cm μPAC columns ...
use guard column Use lower viscosity solvents or higher temperature Use larger dp packing Replace column Reverse solvent flow (Continued) 15 AGILENT SUPPLIES FOR WATERS LC SYSTEMS HPLC Troubleshooting Symptom Type Leaks Leaks, injection valve Leaks, column or other fittings Leak, pump Negative peaks ...
μPAC Neo High Throughput Columns for single-cell to comprehensive proteomic applications Improve your chromatography and analyze hundreds of samples per day, day after day, and do it without sacrificing performance and reproducibility. Low-flow columns for reliable and robust data ...
You'll discover important maintenance procedures and troubleshooting tips. Plus you'll have all the Agilent part numbers you'll need— in one easy-to-use reference guide. "We build our HPLC systems from the ground up and provide our customers with genuine replacement parts for that 'PerfectFit...
HPLC Troubleshooting Symptom Type Baseline disturbance at void time Detector leaks Drifting baseline Ghost peaks Possible Cause Positive/negative – Difference in refractive index of injection solvent Plugged inlet frit Positive direction – Contaminant buildup/elution Positive/negative – Difference in ...
troubleshooting, and maintenance procedures. The new Alliance iS HPLC System has intuitive real-time monitoring of system operational status and uses eConnect-enabled HPLC columns. The injector has a flow-through needle design (split-loop) with reduced carryover. ...