The PL-SCX matrix, being polymeric and macroporous, is stable in most polar mobile phases. The excellent chemical resistance of both the base polymer and cation exchange functionality enables the use of aqueous buffers in the pH range 1–13 without accelerated column degradation or loss of ionic ...
Pre-column exchange is recommended once a week to avoid a contamination of the HPLC column which could lead to a loss in column life time (see figure A 1). A.1. Regeneration of the column Regeneration of the HPLC-column should be done according to the manufacturers instructions. Note: ...
Improving separation efficiency and accelerating elution can enhance sample throughput in many ways (e.g., use of shorter columns, smaller silica particle size and temperature elevation). However, these so called "enhancements" result in fundamental changes in methodology. Developing andvalidating new ...
The ZIC ®-HILIC column can be operated in the pH range 3 to 8, while strongly alkaline solutions and washing with sodium hydroxide should be avoided. The ZIC ®-HILIC column can be heated and operated up to 70 °C.Cleaning and Regeneration If the backpressure increases or a shift in ...
Regeneration method of reversed-phase packing column of HPLC (high performance liquid chromatograph)The invention discloses a regeneration method of a reversed-phase packing column of HPLC (high performance liquid chromatograph). The regeneration method comprises steps as follows: (1), the reversed-...
The following wash cycle is recommended when using the column for a new analyte and application to ensure thorough cleaning and complete conditioning for your subsequent use. Longer-Term Storage Before storing a column, initiate the following cleaning and regeneration cycle. It is imperative to ensure...
TheColumn Swapper™Alternating Column Regeneration System TheLC Sample Peak Recycler™Analytical Recycle HPLC System TheLC-MS Watchdog™Source Contamination Minimizer The2D Multidimensional Column Switching Systemfor LC x LC HPLC Expert HPLC Method Development Consulting (esp. Chiral): ...
Column Cleaning, Regeneration, and Storage After heavy use, your GlycoSep R column may become contaminated with strongly adsorbed sample constituents, which will lead to a loss in column performance. To clean the column flush any salts using at least 20 ml of 5 % methanol or acetonitrile (aq...
The ZIC®-HILIC column can be operated in the pH range 3 to 8, while strongly alkaline solutions and washing with sodium hydroxide should be avoided. The ZIC®-HILIC column can be heated and operated up to 70 °C. Cleaning and Regeneration If the backpressure increases or a shift in ...
If so, see: Reducing Cycle Time for Charge Variant Analysis of Monoclonal Antibodies Alternating Column Regeneration Using an Agilent 1200 Infinity Series Quick-Change Bio-inert 2-position/10-port Valve (publication 5991-4722EN) www.agilent.com/chem/library 52 CHARGE VARIANT ANALYSIS Virtually ...