Connecting the Column to Your System Supelco HPLC columns are provided with zero dead volume threaded end fittings. Flow direction during the column packing process is indicated on the column. Operate the column with the mobile phase flowing in this direction. Before connecting the column outlet ...
If using high flowrates, high column temperatures, or a column with a large internal diameter, for example, and the mobile phase flows through the column without being sufficiently heated, a temperature gradient can form in the cross-sectional direction of the column. (The temperature is lower ...
HPLC column switching technology is the use of multi-channel switching valves to change the direction of the mobile phase or the mobile phase system, so that the eluent enters the secondary column (analysis column) from the primary column (pretreatment column) at a specific time. Because the ch...
(1) Wash entire LC system prior to the column installation, including all flow-lines and sample loop by switching the valve, and then replace the washing solution with the eluent to be used. If desired new eluent has low miscibility/solubility to the eluent of previous analysis, first use ...
Flow Direction The arrows on the column label indicate the flow direction. Begin by connecting the inlet end of the column to the injector or autosampler and gradually increasing the flow rate to its optimum value. Allow the mobile phase to flow from the outlet end of the column into a ...
Cleaning method (1) Removing column inlet blockage (presence of insoluble components) Insoluble components that block the column inlet may be removed by reversing the flow direction, i.e., introducing the eluent from the column outlet, with flow rate at less than half of the recommended flow ra...
mobile phase is pumped in one flow direction through both the separation and guard columns, but the switched valve maintains the same flow direction only through the separation column and reverses the mobile phase flow direction through the guard column, for effectively cleaning the guard column. Mo...
选择性改变 Date 27 Changes in Retention Same Column, Over Time 相同柱子上保留时间改变,超时 l May be cause by: 可能的原因: l Column aging 柱老化 l Column contamination 柱污染 l Insufficient equilibration 不够平衡 l 柱/流动相组成差 l Change in mobile phase 改变流动相 l Change in flow rate...
3. Disconnect any tubing that directs flow through the column heater. Warning: To prevent injury from splashing solvent, wear eye protection and powder-free, nonlatex gloves when handling tubing that can contain solvents. If possible, drain tubing before handling it. 4. Verify that the drip ...
The stationary Column is installed and equilibrated to measurement conditions. Step 5: Samples are then introduced into the flow path. Step 6: The analytes are selectively retained on the downstream Column. Step 7: Upon exit from the column, the now separated analytes are analyzed according to ...