The primary difference between HPLC and LCMS lies in their detection mechanisms and the information they provide. HPLC, while versatile in separation, relies on detectors that measure absorbance, fluorescence, or refractive index changes; it does not provide molecular mass information. LCMS, however, ...
Chromatography additives can make a drastic improvement in both separations and ionization. The level of product refinement is critical to the successfully implementing mobile phase additives into your chromatagraphy and spectrometry applications. CovaChem's LCMS and HPLC additives are some of the most hi...
Conversely, UHPLC demands that the highest quality solvents (UHPLC or LCMS grade) be used and that samples be religiously filtered of particulates. UHPLC, while powerful, is far less forgiving than HPLC in this regard. Beside robustness, capital cost and operating cost for HPLC are lower. ...
Results: After calibration with the commutable material, the median intermethod difference (DEA-AMP) and ratio (DEA/AMP) dropped from 195 U/l to 0 U/l and from 2.47 to 1.00, respectively. When a non-commutable material was used the former became 124 U/l and the latter 1.94. After ...
(total organic carbon or TOC) by using ultraviolet irradiation to decompose organic matter. In particular, in the case of gradient elution, the difference in TOC levels can significantly affect the appearance of ghost peaks. On the other hand, if a fluorescence detector and mass spectrometer ...
[22]. According to the findings of the students’ t-test and F-test, which are displayed in the shown in supplementary material (TableS7), there was no significant difference between the adopted method and the reference one. The chromatogram of laboratory formulated eye drops is shown in ...
Fig. 1. Structures and the main preparation conditions of the saikogenins. 2.2. Instrumentation The UHPLC-MS/MS system used for all the analysis was a Shimadzu LCMS-8060 system (Shimadzu Co., Kyoto, Japan) which consists of a binary pump LC-30AD, a column oven CTO-20AC, a controller...
release from RBL-2H3 cells. Results are present as mean ± SD from triplicate experiment. Mean differences between sample and positive control were analyzed by student’s t-test. *Significant difference comparing tested sample and positive control analyzed by Student’s t-test (p-value <...
All data were recorded and processed using LCMS solution version 3.60, Formula Predictor version 1.2 and Accurate Mass Calculator software (Shimadzu, Kyoto, Japan). 3.7. Prediction of Taxifolin Metabolite Targets The potential targets of the metabolites of taxifolin were predicted using PharmMapper ...
by a JASCO system (PU-2089 Plus quaternary gradient pump) that was equipped with a MD-2018 Plus photodiode array detector (JASCO Europe Srl, Cremella, Italy) and Sedex 85 high-sensitivity LT-ELS detector (SEDERE, Alfortville, Paris, France). The purity of1was verified by1H NMR and LCMS...