结合ChIP-seq和mRNA-seq联合分析的结果,进一步通过非靶向代谢组学和临床血常规数据分析,研究HOXC6调控的代谢途径.结果:体外细胞实验证明HOXC6能促进结直肠癌细胞增殖,克隆形成,调控肠癌的细胞周期向G2期进展;体内动物实验证明HOXC6能促进肠癌细胞在裸鼠体内的成瘤能力和促进肝转移.通过对RNA-seq数据分析,我们发现HOXC6...
由于RNA-seq分析表明HOXC4和HOXC6可能具有冗余功能,因此作者接下来想确定它们在全基因组范围内的结合情况。使用带有Flag标签的HOXC6和HOXC4蛋白进行CHIP-seq分析,HOXC6的CHIP-SEQ分析表明,约73%的HOXC6峰包含典型的富AT的HOX结合基序,该基序位于峰的中心(图2A 上)。然后,作者注释了HOXC6结合位点相对于它们在特定基...
Finally, the ChIP-seq data were used to analyze the carcinogenic mechanism of HOXC6. Bioinformatics methods were used to screen 30 differentially expressed genes in MSS right and left CRC, of which HOXC6 in the right colon cancer was significantly higher than that in the left-sided colon ...