Compressing the flexible sidewalls (12c), and thereby rupture the container (20a), and is mixed with the contents of the sample. Filtered by (16) filter, a reagent to form reagent-sample mixture with the reagent diffuses from the matrix (18) mixture.チュ アルバート イー...
Add the following reagents to the aqueous phase, in the listed order in above table. Place the tube at –20°C overnight to precipitate the DNA from the sample.Note: If you wish to continue with the protocol, place the tube in dry ice or at ...
Can be used to assay for the function of other reagents used in the enzyme digest. If an inhibitor is suspected in the DNA solution, a set of digests comparing experimental DNA, control DNA and a combination of the two may also be performed. In most cases, the presence of an inhibitor...
Secondary peaks exclude the drug substance(s), reagents, internal standards and derivatising agents. Peaks that are due to the mobile phase, sample matrix, excipients and counter-ions can also be disregarded. Any other secondary peak refers to any related substance peak in the chromatogram, apart...
The storage methods for reagents can vary significantly due to variations in reagent sensitivity to environmental factors. Dry storage is the recommended approach for most reagents, as hydrated reagents can potentially undergo adverse reactions that may affect their long-term functionality. Moreover, the...
The use of secondary detection reagents will give you the flexibility to choose from a range of fluorophores to fit into most flow cytometry panels. As multiple secondary antibody molecules will bind to each primary antibody, the signal will be amplified, a great benefit when looking at low dens...
They compared this dye to LC3 and demonstrated that Lysotracker gave similar results, and importantly, was easier to use than LC3. Using flow cytometry and a host of different reagents, it is possible to tease out how your cells may have died. Like the most famous consulting detective once ...
How to use anisole correctlyApr 13,2022 Introduction Anisole is an organic compound with the chemical formula C7H8O with a pleasant anise-like aroma, used in organic synthesis and also as a solvent, fragrance and insect repellent. For organic synthesis, it is also used as solvent, fragrance...
Prepare all reagents as instructed. Add 50 µL of standard or sample* to each well. Add 50 µL of diluted Microparticle Cocktail to each well. Incubate for 2 hours at RT on a shaker at 800 rpm. Wash by removing the liquid from each well, filling with 100 µL Wash Buffer, and...
Nano-secondary reagents can achieve higher resolution and better tissue penetration due to their smaller size. Left: primary antibody (dark blue) & Nano-secondary (green) complex. Right: primary antibody (dark blue) and conventional polyclonal secondary antibody (light blue) complex. Epitope is ...