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Immunofluorescence is compatible with a variety of imaging approaches, including standard widefield imaging, confocal microscopy, lifetime imaging, and super-resolution microscopy[3-4]. The choice of which to use largely depends on the specific application. ...
attempts to use available chelators (deferoxamine) to bind iron ions in viral pneumonia not only did not have a positive effect on the course of the pathological process, but, contrary to expectations, increased mortality (Dolganova and
Testing of the adverse effects of chemicals to humans and the environment relies at present to a large extent on animal models such as rodents and adult fish [72]. It is, however, known that the exposure of animals to chemicals may inflict pain and distress. Hence, there is an increased ...
Zebrafish are primarily of use in pharmacodynamic studies, but even if not being used for pharmacokinetic reasons one needs to be aware of pharmacokinetic issues in zebrafish to interpret studies. Zebrafish are DMSO tolerant and, unlike the invertebrates with their cuticles, readily absorb chemicals....
or no mutations and influenza HA or HN were allowed to bind to their sialic acid receptors on red blood cells (RBCs) at 4 °C, then incubated at 37 °C to permit F activation (Fig.2C). The use of unprocessed F0proteins for this experiment allows specifically for measurement of the...
We use cryoelectron microscopy (cryo-EM) to determine the structure of the phi-particle. We show how the motor domains self-dimerize and are locked in a conformation with weak affinity for microtubules. Disrupting the motor dimer by structure-based mutagenesis drives dynein into an open form ...
To this end, we made use of Grp::cre;Ai32;Grpr::eGFP triple transgenic mice (short Grp-ChR2;Grpr::eGFP mice). These mice express a channelrhodopsin2-eYFP fusion protein (ChR2-eYFP) in the cell membrane of GRP neurons and eGFP in the cytoplasm of GRPR neurons, allowing targeted ...
Any compound that may be considered for therapeutic use has to be tested as a co-treatment and at various times post APAP treatment using clinically relevant doses. In addition, any potential effect of the intervention on the different phases of the APAP-induced injury mechanism needs to be in...
To accomplish this, we used a biorthogonal non-canonical amino acid tagging (BONCAT) pulse-chase approach.27 Specifically, the core of our approach relied on the use of the non-canonical amino acid called azidohomoalanine (AHA) that functions as an analog of methionine and possesses a reactive...