The higher reaction temperature helps denature regions of strong RNA secondary structure, which can cause RTs to stall and limit cDNA size (6–7). For this reason, AMV RT is often used to reverse transcribe RNAs with strong secondary structure. Like other RTs, AMV RT is compatible with gene...
and complementary in nucleotide sequence to the template strand of the DNA. The other strand of DNA, referred to as either the nontemplate strand or the coding strand, has the same 5' to 3' orientation and sequence as the mRNA except that T is present...
How do I transcribe DNA to mRNA and mRNA to protein? Explain chromatin remodeling. What is it and what does it do to DNA? What is the first step in replicating DNA in chromatin form? How are DNA, RNA, and proteins related in the cell?
RNA-dependent RNA Polymerases (RdRP), found in viruses such as SARS-CoV-2 and influenza, are employed by the pathogens to transcribe RNA from viral RNA templates. Viruses localized in the cytoplasm of a cell cannot access the host RNA polymerase and therefore encode their own polymerase to pro...
These businesses usually charge by the minute of audio that needs to be transcribed and usually market the niche fields of interest they are able to transcribe. What would you record for transcription? There are any number of things that you may want to record for transcription later. The most...
Transcription - How to do it ?Transcribe the following DNA strand into two single stranded pieces of RNA: A A T G C C G T A T A G C T C A T G C T A T T A C G G C A T A T C G A G T A C G A T please explain how to do this ...
To create an enzyme, the cell must first transcribe the gene in the DNA into messenger RNA. The transcription is performed by an enzyme called RNA polymerase. RNA polymerase binds to the DNA strand at the promoter, unlinks the two strands of DNA and then makes a complementary copy of one...
speckles, which store cellular materials needed for RNA splicing, nucleoli, where ribosomes are formed, and a new kind of droplet Young's lab discovered in 2018 called a transcriptional condensate. These new condensates bring together all the different proteins needed to successfully transcribe a ...
DNA sequence, called the operator, that is recognized by a transcription regulator. When this regulator binds to the operator, it blocks access of RNA polymerase to...
Therefore, to explain gene fusion, we argued that the presence of the two coactive genes at the same time and place, with the chromatin open at both loci due to transcription, enables various downstream mechanisms, such as reverse transcription of the RNA (perhaps aided by trans-splicing), ...