To keep cells in optimal culture conditions and actively growing, it is necessary to renew the growth medium and to subculture them at regular intervals. Change of culture medium can take place several times in the log phase dependent on the cell type. The best time to subculture cells is be...
The COS-7 cells were cultured in DMEM (Gibco, USA) containing 10% FBS (Gibco, USA) and antibiotics (100 units mL−1penicillin and 100 µg mL−1streptomycin) in a 5% CO2humidity incubator at 37 °C. The cells were subcultured every two or three days. ...
In this state, most nuclear genes were switched off in these cells, and their nuclei were characterized by being compact with highly condensed chromatin and a small nucleolus. Cultured alfalfa cells in stationary phase (11 days without subculture) suspension were transferred to fresh media and the...