Once you’ve prepared your samples and reagents as directed in the previous sections, it’s time to run your Quantikine ELISA. We recommend that all samples, controls, and standards be assayed in duplicate. Remove excess microplate strips from the plate frame, return them to the foil pouch co...
Degradation of cells in a population can occur due to natural cell death, in response to therapeutic testing, or as a result of the experimental procedure. Whatever the cause, when cells become dead or damaged, they leave behind cellular debris—both unhealthy cells and ...
How Automating Elisa Assay Processing WorksHamilton Robotics
HOW TO CHOOSE THE RIGHT ELISA KIT As a general rule, before purchasing an assay, always read the protocol booklet (instructions for use – IFU or package insert) in detail. This should ensure that the kit will be suitable for your requirements. Check out the following: ...
ELISAs are usually run in 96-well microplates coated with a capture antibody specific for the analyte of interest. While they are excellent analytical tools, there are some disadvantages to ELISAs, especially when they are a frequently used assay in your lab. Disadvantages of ELISAs include ...
The standard curve of ELISA generally uses special curve fitting tools, such as: CurveExert 1.4. CurveExpert 1.4 is a comprehensive curve fitting system for Windows. XY data can be modelled using a toolbox of linear regression models, nonlinear regressio
No. 57 – 2022 >> Fit for Cell Culture: Expand Your Know-How > Data digitalization increases the efficiency of your research > Smart sample separation to streamline your workflow > Sustainable: pipettes with the ACT® Label Application Notes Standardized thawing of stem cells using the ...
(RT-PCR assay) can also detect norovirus in food, water, stool samples, and on surfaces. These tests isolate and replicate the suspected virus’s genetic material for analysis. An ELISA can also be performed, which detects antigens; they are easier to perform than RT-PCR, but less ...
Reducing the time taken to run qPCR assays on today's qPCR cyclers is rather straightforward and requires no specialised reagents or instruments. As the first article in a new series of short technical reports, I demonstrate that it is possible to reduce significantly both denaturation temperatures...
8 Like fluorescence intensity, this method can efficiently be run on a plate reader with fluorescence polarization detection. See also: AN326: Fluorescence polarization-based RNA synthesis assay Compound screening based on interaction assays Interaction assays are of utmost importance for the screening ...