SYBR Green I, to your master mix during qPCR setup. This fluorescent dye intercalates into double-stranded DNA sequences during the extension phase, where it shows a strong increase in fluorescent signal. Measuring
Hazard and risk assessment of nanomaterials (NMs) face challenges due to, among others, the numerous existing nanoforms, discordant data and conflicting results found in the literature, and specific challenges in the application of strategies such as grouping and read-across, emphasizing the need for...
7. Click the Uninstall button. A confirmation window will come up. accept the uninstall by pressing Uninstall. Advanced Uninstaller PRO will automatically remove StepOne Software v2.1. 8. After uninstalling StepOne Software v2.1, Advanced Uninstaller PRO will ask you to run an additional cleanu...
Genomic DNA or plasmids containing the gene of interest can be used as standard for validation of assays for DNA analysis, preferably after excising a fragment containing the target sequence to remove interfering supercoiling [26]. Still another option is to use synthetic templates (e.g. gBlocks...
We washed the cells to remove the arresting reagent and auxin, thereby allowing the cells to reenter the cell cycle and to recover Sen1-AID expression. Cells were plated to monitor cell survival (Figure 2C). Cell death occurred in Sen1-AID triple cells after transient Sen1-AID depletion ...