You can also use UV light to further reduce DNA contamination. Cleaning Pipettes for RNA Work It is especially important that your clean your pipettes before doing any RNA work, as any RNase contamination will
Test the enzyme, reaction buffers and gel loading buffer for DNA contamination. Presence of a second restriction enzyme Detect a second activity by repeating the unit activity assay or test a known DNA substrate with a defined number of cut sites for the enzyme. Volume of reaction decreased ...
using the interphase and organic phase for genomic dna and protein purification, respectively. 7 nowadays, an even more streamlined method exists to remove the need for phase separation and reduces the ≥ 100-minute trizol ® phenol-chloroform rna extraction down to only 7 minutes. the direct-...
Pipettes are so commonly used in the laboratory that it can be easy to take them for granted. Without proper care and maintenance, pipettes can become less accurate and even cause sample contamination. The best way to prevent this source of error and frustration is to clean and maintain pipett...
For instance, DNA sequencing can specifically target variation in the bacterial 16s rRNA gene to taxonomically identify organisms within the gut microbiome. Transcriptomic studies can capture gene expression signatures from specified cells of interest by combining RNA-seq with cell-specific isolation ...
ALH robotic systems are designed to automate the precise handling of liquids, facilitating a wide range of applications such as sample preparation, DNA/RNA isolation, genetic or protein-based diagnostics, and high-throughput screening [[1], [2], [3], [4], [5]]. While each application ...
In addition, since the UMIs label each DNA fragment at the beginning, bioinformatics solutions will enable you to remove PCR duplicates, distinguish molecular duplicates, and consequently obtain a valid count of the starting molecules. This provides accurate coverage information of the genomic regions ...
RNA. Meanwhile, the NUC lobe is composed of three domains: the "Resolvase, UvrC C-terminal" (RuvC), the "His-Asn-His" (HNH), and the protospacer adjacent motif (PAM) interacting domains. The RuvC and HNH domains are responsible for cleaving each strand of DNA, while the PAM ...
Fungi have been companions of mankind for millennia. Mushrooms inspired our eating culture, and yeasts and filamentous fungi were developed into highly efficient cell factories during the last 100 years to produce many products utilized in different indu
TECHNICAL NOTE Collibri DNA library preparation kits How to prevent index hopping Summary • Index hopping causes misassignment of sequencing data from one sample to another • Use of unique dual indexes (UDIs) mitigates concerns about index hopping • Invitrogen™ Collibri™ DNA library ...