cell. It is different from flow cytometry in the way that it provides unique characterization versus merely counting and sorting cells. It is common for the two principles to work in a co-characterization type process to offer a complete qualitative and quantitative approach to flow cytometric ...
3. Check the stability of the run. Plot a time vs a scatter plot to see how even the flow was during the run. Using a plot like this will help eliminate artifacts caused by poor flow. Check out the plot below. The left plot shows good, even flow, while the right plot shows poor ...
Full-spectrum cytometry also allows for closely related dyes, that would not work together in traditional fluorescent cytometry, to be used in panels on these spectral cytometers. Take, for example, AlexaFluorⓇ 647 and APC, as shown in this spectral plot and a traditional 660/20 BP filter....
Comparing to a generic two-state model, our models can distinguish class I from class II MHCs and correlate their structural parameters with the TCR/pMHC’s potency to trigger T cell activation. The models are tested by mutagenesis using an MHC and a TCR mutated to alter conformation changes....
That’s about it! We hope you now have an appreciation of what doublets are, in terms of the instrumentation, and how and why to make sure they are excluded when analyzing a data set. To learn more about How to Perform Doublet Discrimination In Flow Cytometry, and to get access to all...
However, in FACS, each cell is only “a dot in a plot”. Features like affinities, secretion dynamics, and cell–cell interactions over time are not easily attainable by FACS. Recent advances enhanced the optical resolution of flow cytometry, such as polychromatic flow cytometry (PFC)8 and ...
The first few tubes looked acceptable, but eventually, cooper- ation between your handwriting, the pen, and the smooth surface of the tube began to falter. You may have been able to read your artwork – but only you. The SafeCode System for Eppendorf consumables is based on multi-level ...
The transcriptomes of several cyanobacterial strains have been shown to exhibit diurnal oscillation patterns reflecting the diurnal phototrophic lifestyle of the organisms. The analysis of such genome-wide transcriptional oscillations is often facilitate
Authors basically admit to have been manipulating their flow cytometry data by arbitrarily tweaking the settings for each sample, instead of adhering to same gating setup throughout data acquisition (read about arelated case here). Yet the journal (published by the International Society for Stem Cel...
Comparing to a generic two-state model, our models can distinguish class I from class II MHCs and correlate their structural parameters with the TCR/pMHC’s potency to trigger T cell activation. The models are tested by mutagenesis using an MHC and a TCR mutated to alter conformation changes....