Step 1: Make a concentrated stock solution Prepare a concentrated stock solution of the standard by weighing the solute and transferring it to a volumetric flask with solvent.See this step-by-step guide for making aqueous solutions. Step 2: Make the standards for the calibration curve Perform a...
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When determining the volume of the flask, the student did not fill the flask completely with water and left approximately 1 mL of air at the top of the flask prior to weighting it. What will be the effect on the measured density of the...
Add 1 ml of 100 mM EDTA. Bring the volume up to 100 ml with a volumetric flask and store at 2-8°C. Ideally, protease and phosphatase inhibitors should be added on the day of use (100 μl each of aprotinin, leupeptin, and pepstatin; 500 μl each of PMSF, Na3VO4, and NaF). ...
The accuracy of a graduated cylinder is higher because the graduations on the cylinder make it easier to more precisely fill, pour, measure, and read the amount of liquid contained within. Why is a pipette accurate? Why are Volumetric pipettes more accurate? volumetric pipet more accurate becaus...
How would you prepare 100 mL of a 155 ppm solution of urea in water? How much of a 0.35 M solution of Fe2+ is needed to make a solution of 1.5 mM Fe2+ in a 100 mL volumetric flask? A solution is labeled 100 ppm MgCl2. What...
Both dilutions would involve pipettes and volumetric flasks. Here, the serial dilution uncertainty rises to 6× larger than the best 1:50 dilution (0.39% versus 0.06%). To understand the nuances of the process dilution you really should to read this column (4). Summary In this installment ...
How do you use titration to measure pH? To find the pH at the halfway point of the curve, divide the volume of base needed to reach the equivalence point by 2, and read off the corresponding pH from the titration curve. Determine your experimental value of Ka for acetic acid. Why do...
This solution is a standard against the sodium chloride solution (NaCl). Experimental Work – Steps: Take 50mL of sodium chloride sample in a conical flask Add 1.0mL of potassium chromate to the flask. This potassium chromate serves as an indicator Indicator An indicator, parti...
Add the solid solute into the beaker first before adding the solvent. This will avoid adding excess solvent to the solution. You should allow dry solute to dissolve in solvent first before adding to the total volume. Pour solution into a graduated cylinder or volumetric flask and add solvent ...