Determining the needed target species is typically straightforward; it is the species from which the primary antibody was derived. If one is using a rabbit polyclonal primary antibody, then an anti-rabbit secondary antibody is needed. If one wants to detect endogenous antibody ...
11. Add a certain volume of rabbit antibody to 500 μl of total protein. The dilution ratio of the antibody depends on the abundance of the target protein in different cell lines. 12. Shake the antigen-antibody mixture slowly at 4°C overnight or at room temperature for 2 hours. For ki...
The secondary antibodies used in various assays are typically polyclonal. A secondary antibody should be raised against the primary antibody’s host being used. For instance, if a primary antibody is produced from rabbit, then the secondary antibody should be ‘anti-rabbit’. This information should...
During a single flight, there is enough nitrogen to open the valves and purge the lines 10 times! Either one or both of the OMS engines can fire, depending upon the orbital maneuver. Each OMS engine can produce 6,000 lb (26,400 N) of thrust. The OMS engines together can accelerate ...
C AIF Rabbit (pAB) Apoptosis inducing factor Milipore, AB16501; Temecula, CA 1:250; C mAB monoclonal antibody, pAB polyclonal antibody, C citrate buffer pH 6, E ethylenediaminetetraacetic acid buffer pH 9.0, prot Protease XXIV; 0.03 %, 15 min also used to determine the number of axons wi...
The first thing to notice on a topographical map is the title. It is found in the top right hand corner of the map: The title for this particular map is, "Sunset Crater West Quadrangle." At the corner, but in smaller print is another title called Strawberry Cheater. That is the title...
The lower part of the light wave passes between the layers first, so it speeds up an instant before the upper part. The light that would ordinarily go straight to the ground bends upward and travels to your eyes. The effect is that you see the image of the car twice — once on top ...
Liquid nitrogen is fairly messy and difficult to handle. So why is nitrogen a liquid while carbon dioxide is a solid? This difference is caused by the solid-liquid-gas features of nitrogen and carbon dioxide. When you put dry ice with water it will produce smoky fog like seen here — ...
(anti-active MAPK polyclonal antibody), phospho-p38 (Promega, Madison, WI), PAR (Trevigen), γH2AX (Millipore), and GAPDH (Ambion), and detection was performed by enhanced chemiluminescence (34) using species-specific HRP-conjugated goat anti-mouse (1:10,000 dilution) or goat-anti-rabbit (...
The phenotypic and functional diversity of B lymphocytes, their regulatory roles independent of antibody production, and the molecular events that make this lineage unique are also considered. Finally, perturbations in B-cell development that give rise to certain types of congenital immunodeficiency, ...