50x TAE buffer recipe The recipe below can be used to prepare a 50x 1 L stock solution of TAE buffer. From this, a 1x working solution can be prepared. Reagent Weight/Volume Final concentration Tris base 242 grams 2 M Glacial acetic acid 57.1 mL 1 M 0.5 M EDTA, pH 8.0 100 mL 0.05...
Prepare a Working Solution of TAE Buffer The working solution of 1x TAE buffer is made by simply diluting the stock solution by 50x in deionized water. Final solute concentrations are 40 mM (millimolar) Tris-acetate and 1 mM EDTA. The buffer is now ready for use in running an agarose gel...
Here’s the best way to solve it. Solution Share View the full answer Previous question Next question Transcribed image text: Make up 1000 mL of 1XTAE solution using 50X TAE. How many mLs of 50X is needed? 5 10 20 25 Question 10 ...
Calculate how many grams of Na2EDTA dihydrate (Na2C10H14N2O8 2H2O, molar mass = 372.24 g/mol) will be needed to prepare 500.0 mL of an EDTA solution that is approximately 0.010 M. Calculate how many grams you need to make a 20 mM...