double-stranded DNA libraries. Subsequently, the transcriptomic data are sequenced using NGS platforms, and the information is assigned to individual cells. By clustering cells based on similarities in gene expression profiles, cell types can be annotated using known marker genes. Additionally, cells ca...
Index hopping is the incorrect assignment of libraries to a different index. It has a limited effect. Follow these best practices to minimize it.
The easiest and most relable solution is to ensure that all libraries in the sequencing pool contain UDIs. UDIs require that index 1 and index 2—the specific 8-base sequences used to distinguish the DNA sequence of a unique sample—are used only once per sample (Figure 1). The second ...
P. Bryan Heidorn (2011) The emerging role of libraries in data curation and e-science [d] David J. Hess (2011) To tell the truth: on scientific counterpublics [d] Garry W. Jenkins (2011) Nongovernmental organizations and the forces against them: lessons of the anti-NGO movement [u] T...
Annotations can be made on different levels (from the document level to individual words) and with regard to different aspects, like the semantic role of a sentence or a legal assessment. Some types of annota- tions are more prone to disagreement than others. 3. Pool of annotators, ...
Index hopping is the incorrect assignment of libraries to a different index. It has a limited effect. Follow these best practices to minimize it.