Do you know how to use a pipette? These tips on how to pipette will instantly improve your pipetting technique and help you to correctly use a micropipette.
Set the pipette to the desired volume. Depress the plunger completely – past the first stop to the second (blowout) stop. Immerse the tip in the liquid, and slowly release the plunger to full extension. Dispense by pressing to the first stop. ...
Each pipette has an optimal volume range, which is typically 35-100 % of the nominal volume for an air displacement pipette. Operating below this range results in too much air in the cushion, which can lead to inaccurate results. This means that staying within this range is cr...
Small volumes (50-100 mL) can be washed down the sink. Large volumes should be collected as hazardous waste. Note: if step 5 is necessary, add a small amount of the piranha solution to the waste container and inspect for any reaction. If there is no reaction, continue to pour the rema...
After removing the pipette, gently aspirate just a tiny bit of air to create an air bubble (approximately 0.5 mL) in the pipette and allow the air bubble to travel up the pipette to mix the acrylamide on its way up. Slowly pipette the gradient solution into the gel cast. Beats the grad...
The dilution factor is used to calculate the number of cells in the original cell culture. In our example, an average of 50 colonies was counted on the plates obtained from the 1:10,000 dilution. Because only 0.1 mL of suspension was pipetted on the plate, the multiplier required to reco...
A pipette isaccurate to the degree that the volume delivered is equal to the specified volume. ... A pipette can be consistently inaccurate but this inaccuracy could be very precise, for example if a pipette reads consistently low. Is a pipette the most accurate?
Low-surface tension fluids, particularly those with high vapor pressure, also pose a significant challenge during pipetting as they tend to drip from pipette tips during volume displacement [51]. This dripping can lead to inaccurate volume measurements and potential sample loss, compromising ...
But you don't want to make 0.13 aliquots because it is too small. Make a dilution to accommodate If a researcher tries to make a dilution of 1000 pg/mL directly from a starting stock of 1 \mu g/mL. What volume in mi...
This beautiful dance was caused by the local input of latent heat at the freeze front, causing a strong Marangoni flow capable of breaking off small ice crystals. Isothermal bubbles therefore freeze very efficiently due to hundreds of fronts growing in tandem and interlocking together. When a ...