To study the function of a specific gene and its potential implications in angiogenesis, the most straightforward approach would be to either overexpress or silence it in ECs. We and others have tested multiple protocols for either transfection and transduction of primary ECs in vitro and in vivo...
With the intensification of the greenhouse effect, a series of natural phenomena, such as global warming, are gradually recognized; when the ambient temperature increases to the extent that it causes heat stress in plants, agricultural production will in
As already explained, EVs play a multifaceted role in cancer progression, influencing various stages from tumor growth to metastasis and also contribute to the immune system escape. Their diverse composition, reflecting both their biogenesis machinery and the dynamic cellular environment, serves as a mo...
use Xenopus embryos to heterologously overexpress the rat VAChT in this first functional study. Their successful overexpression of the VAChT may encourage others to try this approach with other vesicular transporters or synaptic proteins. Investigators could potentially fashion synapses emphasizing whatever ...
With the intensification of the greenhouse effect, a series of natural phenomena, such as global warming, are gradually recognized; when the ambient temperature increases to the extent that it causes heat stress in plants, agricultural production will in
RNA formulating is recognized to happen in innumerable cancers such as the GC. A study related that gastric tumors that both of them overexpress ADAR and down regulate ADARB1 have the arrant prognosis [102]. Moreover, inaccuracy in RNA formulating at ADAR-peculiar positions little by little ...
The researchers found it has two novel responses to extreme cold temperatures: it can switch its metabolism from respiration to ethanol fermentation as its main pathway, and can overexpress molecules called small non-coding RNAs (sRNAs) that help regulate which genes are expressed after transcription...
: Inflammatory microcrystals alter the functional phenotype of human osteoblastlike cells in vitro: synergism with IL-1 to overexpress cyclooxygenase- 2. J Immunol 2002, 168:5310–5317. PubMed CAS Google Scholar Liu R, Liote F, Rose DM, et al.: Proline-rich tyrosine kinase 2 and Src ...
nigerto heterologously overexpress a non-ribosomal peptide synthetase (NRPS) fromFusariumspp. in several g/L amounts [55]. This proof of principle experiment, which utilized the highly optimised and titratable synthetic Tet-on gene switch [25] to produce the antimicrobial cyclohexadepsipeptide ...
An alternative approach to gene ablation is overexpression, gain-of-function. Plasmid-based overexpression systems have been used for decades to overexpress specific coding genes [178]. Plasmid-based over-expression of lncRNAs is possible but is limited by the size (max size: 6-8 kb) of the...