In order to examine the function of the identified proteins in acid tolerance, we developed the recombinant E. coli strains Ec-g (expressing glyceraldehyde-3- phosphate dehydrogenase), Ec-a (ATP synthase subunit a), Ec-s (secretory protein), Ec-p (NADH dehydrogenase), and Ec-m (...
By this measure, our global alignment above gives us a score of2 matches minus 7 mismatches = -5. We can improve this score if we take into account that each particular strand may have insertions or deletions i.e. we mayshift the nucleotides (or characters)in each particular sequence to f...
auxin is indispensable to steer the cell division plane orientation of new organ-defining divisions. We propose a dual, spatiotemporally distinct role for auxin during lateral root initiation. In the endodermis, auxin releases constraints arising from cell-to-cell interactions that compromise the pericy...
/5Phos/AGACAATAACCCTGATAAATGCTTCAATTCATGTCCAGCCCAAACTCTG; SM105, AACCAATGGACCATTTAACCAATATTCAAATATGTATCCGCTCATG; SM129, GCAAGCTTATGCAGACGGCTCTGATGCCGCATAGTT; SM130, /Phos/CGTCTGCATAAGCTTGC; SM142, AAGCCAGCCCCGAC; SM142C, GTCGGGGCTGGCTT; SM132, AAATAAACATAAAGTAAGTAAGTATAAGGATAATAC...
Antisense: 5′-CTAGATTTCCAAAAAGCCAGAATTCTACCCTGGAAACGGACAGCACACGTTTCCAGAGCAGAACTCTGGCGGG-3′ NONO shRNA-#2 Sense: 5′-GATCCCCCAGGTGAAGTCTTCGTTCGTACGTGTGCTGTCCGTATGAATGAAGACTTCGCCTGTTTTTGGAAAT-3′ Antisense: 5′-CTAGATTTCCAAAAACAGGCGAAGTCTTCATTCATACGGACAGCACACGTACGAACGAAGACTTCACCTGGGG-3′...
In addition, students may focus on the external rewards in test-based situations and fail to develop the ability to self-motivate and to focus intrinsically on the task [12]. The long-term intention of rewards must be that extrinsic motivation will stimulate interest in a new area, which ...
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Moore4, Eric A. Hendrickson3 & Dale A. Ramsden1 Nonhomologous end joining (NHEJ) can effectively resolve chromosome breaks despite diverse end structures; however, it is unclear how the steps employed for resolution are determined. We sought to address this question by analysing cellular NHEJ of...
In more than 400 coastal systems covering a 245,000 km2 area, hypoxia has been recognized as a key stressor in many aquatic ecosystems, especially along populated and developed coasts [3]. Chesapeake Bay, the largest estuary in the United States, is prone to hypoxia due to its linear shape...