I am processing images and I have two files for 1 image, named img32_6.xlsx and img32_6-bg.xlsx. I need to normalise values from img32_6.xlsx with values from img32_6-bg.xlsx, as seen below. And I have written this to process a pair of files "img32_...
To normalise the gene expression levels to the control group average so the control group average value will be ‘1’, make a new column and divide all of the gene expression values for all the samples by the control group average gene expression value. Then average these values for the gro...
"One thing I've learned from travelling is that we often learn things a certain way in our own countries, and we normalise those behaviours and perspectives. But when you travel to a different country, you gain a new outlook. It broadens your thinking, allowing you to understand things ...
The Office data scientists experiment with multiple different models and variations; the best model then gets stored back into Azure Data Lake and downloaded into AML using theONNX Runtime(open-sourced by Microsoft) to run in production without having to be rebuilt. “...
Basically, ∆Ct is the difference in Ct values for your gene of interest and your housekeeping gene for a given sample. This is to essentially normalise the gene of interest to a gene which is not affected by your experiment, hence the housekeeping gene-term. Using the delta-delta Ct for...
If you have more than one reference/housekeeping gene, it may be worth checking out the guide onanalysing qPCR data with numerous reference genes. This approach is very similar to the Pfaffl method, with a slight difference in that it can handle two or more reference genes to normalise to....