Hazard and risk assessment of nanomaterials (NMs) face challenges due to, among others, the numerous existing nanoforms, discordant data and conflicting results found in the literature, and specific challenges in the application of strategies such as grouping and read-across, emphasizing the need for...
Using a 0.30 M phosphate buffer with a pH of 6.5, you add 0.79 mL of 0.53 M NaOH to 41 mL of the buffer. What is the new pH of the solution? (Give your answer to three significant figures.) Calculate the amounts of 1.0M NaOH...
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Hsp90 is an essential molecular chaperone responsible for the folding and activation of hundreds of ‘client’ proteins, including the glucocorticoid receptor (GR). Previously, we revealed that Hsp70 and Hsp90 remodel the conformation of GR to regulate l
Analogous to a photocopier, we developed a DNA microarray copy technique and were able to copy patterned original DNA microarrays. With this process the appearance of the copied DNA microarray can also be altered compared to the original by producing cop
Eluted protein was diluted to 50 mL in Ion Exchange Buffer A (20-mM HEPES, pH 8.0, 25-mM NaCl, and 2-mM dithiothreitol (DTT) and loaded onto a tandem 1-mL HiTrap Q HP (GE Healthcare)/1-mL HiTrap SP HP (GE Healthcare) column setup. The tandem column setup was then disassembled...
Drought negatively affects crop growth and development, so it is crucial to develop practical ways to reduce these consequences of water scarcity. The effect of the interactive potential of compost (Comp), mycorrhizal fungi (AMF), and carbon nanoparticles (CNPS) on plant growth, photosynthesis rate...
Link to this page: Facebook Twitter References in periodicals archive? The incorporation of glucose into lipids in adipocytes was studied in plastic tubes at 37 [degrees]C withKrebs-Ringer buffer, pH 7.4, containing 3% BSA, 10 mM HEPES, 0.5 [micro]Ci of [[U.sup.14]-C]glucose per ml ...
Where needed, aliquots of PI3K were thawed on ice and diluted into stabilizing buffer (125 mM NaCl, 20 mM HEPES (pH 7.2), 25% glycerol, 4 mM TCEP, 0.05% CHAPS, and 100 μg mL−1 BSA) that maximizes their stability on ice until they are diluted, just before use, to the final ...
The h0 residues I82 and I83 make additional interactions with the Bax groove. Bid D95 forms a salt link to Bax R109 (cyan). Other Bax side-chains shown (cyan) are R94 (close to Bid I83) and I66 and Y164 (both close to Bid M97). See also Figure S2. Notably, the BidBH3 ...