7AdoMet is considered stimulating, but not required, for all the Type III enzymes (10). 8ATPase activity has been previously reported as <1% compared to Type I restriction activity and therefore ATP was regarded as a cofactor rather than a substrate. However, more recent evidence with EcoP15...
If you diluted 1 mL of a sample into 9 mL of water, then took 1 mL of that and diluted into another 9 mL of water, and did that another two times, what is fold the dilution factor? How many milliliters of water wou...
Whether you want to snazz up your kitchen or protect a stainless steel refrigerator from fingerprints, a cover for your refrigerator door handleis an easy at-home project that requires no advanced sewing skills. This project will help you make a door handle cover that is 24 inches long and ...
How much of the first dilution should be made to perform the following dilution: 300 mL of a 1:5 dilution? You have been asked to make a 1:2 dilution with a final volume of 250 L. What sample volume will you need? Include th...
Influenza virus can escape most antibodies with single mutations. However, rare antibodies broadly neutralize many viral strains. It is unclear how easily influenza virus might escape such antibodies if there was strong pressure to do so. Here, we map al
To calculate the reaction efficiency of your assay, you need to set up a 10-fold serial dilution of an undiluted sample with a known amount of template DNA. After running a qPCR, create a standard curve with the log of the starting quantity on the x-axis and the Ct values on the y-...
An aliquot of 100 µL from the stored resuspended RBCs in sub-study (i) were spread on a plate count agar and if needed, ten-fold serial dilutions of the sample to 10–2, 10–4 and 10–6 with saline were performed; from which 100 µL was spread onto a plate count agar (PCA...
subtilis spores without mechanical homogenization with estimated 3.75 x 108 CFU input; plate shown is a 100-fold dilution. (C) B. subtilis spores with an estimated 3.75 x 108 CFU input lysed with high speed instrument for 5 minutes at 6.5 m/s (1 minute interval, 5 minutes rest).Protocol...
pickle them when the pores/tubes are still white or just beginning to yellow. Although I harvest a lot of penny buns each year, such a small proportion of them are crisp and bug-free, that I tend to keep them for usingraw in salads– about the nicest thing you can make with ...
4.You perform 7steps of a 1:10 (10 fold) serial dilution on a 24 hr growth ofliquidbacteria culture. You plate100 microliters of the last two dilution tubes and find that the plate made from the most dilute tubegivesyou 172colonies. What is the concentration of the bacteria (cells/ml...