TBE is generally more expensive than TAE and inhibits DNA ligase, which may cause problems if subsequent DNA purification and ligation steps are intended. With the three simple steps that follow, learn how to make TBE buffer. It shouldn't take more than about 30 minutes to create. What You ...
Given that the buffer is easy to make and the steps can be carried out quickly, making more than one batch at a time shouldn't be particularly time-consuming or difficult. Using the instructions below, it should take just 30 minutes to make the TAE buffer. What You Need for the TAE B...
How Do You Make a Buffer? A buffer is made by mixing a large volume of aweak acidorweak basetogether with its conjugate. A weak acid and its conjugate base can remain in solution without neutralizing each other. The same is truefor a weak baseand itsconjugate acid. How Do Buffers Work?