3V5 strain was created by mating a BY4741 and a BY4742 anchor-away GAL4-3V5 strain that were constructed by the CRISPR–Cas9 approach described above using a repair template created by a PCR product from genomic
Plasmids encoding HPIV3 HN and F were generated through site-directed mutagenesis of a previously constructed pCAGGS mammalian expression vector53 and sequenced via Sanger sequencing prior to experimental use. Transfections with plasmids were performed in HEK293T cells (ATCC) using Lipofectamine 2000 per...
was invented based on the Sanger sequencing principle. In this method the sequencing reaction will be terminated not at the end of the template, but where the protein sits on the DNA, allowing scientists to pin-point the binding site (4). A spin off of that ...
Is the inner retina trained enough to interpret the signal? In diseases such as early onset retinal dystrophies (EORDs), where visual impairment manifests in early childhood, does amblyopia play a role? If so, how does the brain process the new input information? For instance, the ...
CTTV scientists are combining their expertise to explore and interpret large volumes of data with the aim of improving our ability to define the biological targets in a range of diseases. The Wellcome Trust Sanger Institute is contributing its unique understanding of the role of genetics in health...
Despite all these positives, a major obstacleblocks thewidespread adoption ofwhole genome sequencingagainstdrug-resistant bacteria. Currently there are few tools to automatically analyse and interpret sequenced data from clinical samples so that it can be understood and clinically applied by non-specialists...