If you really want to use the absolute IC50,here are instructionsfor fitting a curve to find it. Incomplete dose-response curves Any attempt to determine an IC50 by fitting a curve to the data in the graph above will be useless. A curve fitting program might, or might not, be able to ...
Nonlinear regression can determine a drug’s potency (EC50 or IC50), find best-fit values, compare results from different experiments and interpolate unknowns from a standard curve. For example, you can study the effect of treating cells with an agonist in the presence and absence of an antago...
Error bars in (B), (C) and (D) represent SEM of triplicate data used to calculate the representative IC50 values in (A). (E) Location of BimBH3 ‘h0’ and h1 residues in the proposed alternative Bax binding site, the α1/α6 interface (Gavathiotis et al., 2008). The model ...
We note that the IC50 values reported here for IGFs binding to holoIGF-1R differ from those reported by, for example, Macháčková et al. (2019) yet broadly concur with those reported earlier by Surinya et al. (2008); the source of such variation is unclear but may relate to the ...